Cerebrospinal Fluid following Cerebral Ischemia Accelerates the Proliferation of Bone Marrow Stromal Cells in vitro
スポンサーリンク
概要
- 論文の詳細を見る
The central nervous system in the embryo develops around the cerebrospinal fluid (CSF), which regulates cell proliferation and differentiation. Neurogenesis has been also reported in the subventricular zone (SVZ), which is close to CSF, after stroke in rats. In this study, CSF extracted following stroke in rats was added to bone marrow stromal cell (MSC) culture in vitro, and the proliferation and differentiation of MSCs were studied. Primary cultures of MSCs were obtained from 7-week-old Lewis rats and incubated in a plastic tissue culture flask. CSF was retrieved from other rats 48 hrs after 0, 15 and 75 min after middle cerebral artery occlusion (MCAO). CSF from these three groups were added to respective MSC culture solutions, and the cells were then incubated for 72 hrs. Western blots of the extracellular signal-regulated kinase-1 and -2 (Erk1/2) were obtained just after the CSF induction. The expressions of CD34, CD45, CD90 and CD108 were assessed by flow cytometric analysis. The proliferation of MSCs was accelerated by the addition of post-stroke CSF, especially in the 15-min MCAO, in a dose-dependent manner. The morphology and surface antigens of the cells were maintained in all groups. Phosphorylated-Erk1/2 was elevated in all the CSF-treated groups, although this effect was more enhanced in the 15-min MCAO group. Our data indicate that the addition of post-stroke CSF to the primary medium stimulated the proliferation of MSCs, and that these MSCs maintained their characteristics through the p-Erk1/2 pathway. These results suggest that use of post-stroke CSF as a component of culture media could facilitate the autologous transplantation of MSCs.
著者
-
Shigemori Minoru
Department Of Neurological Surgery Kurume University Medical School
-
Harada Hideki
Department Of Anesthesiology Kurume University School Of Medicine
-
YAMASHITA SHINE
Department of Neurosurgery, Kurume University School of Medicine
-
HARA Masato
Department of Anesthesiology, Kurume University School of Medicine
-
Kikuchi Kiyoshi
Department of Chemistry, Faculty of Science, Kyushu University
-
HARA MASATO
Department of Anethesiology, Kurume University School of Medicine
-
ORITO KIMIHIKO
Department of Neurosurgery, Kurume University School of Medicine
関連論文
- Detection of Single Copy Genes by Two-Pass Tyramide Signal Amplification Fluorescence in situ Hybridization (Two-Pass TSA-FISH) with Single Oligonucleotide Probes
- Olfactory neuroepithelioma : An immunohistochemical and ultrastructural study
- Diffuse Axonal Injury and Early Intracranial Sequelae in Severe Head Injury
- Multiple Primary Brain Tumors of Different Histological Types : Report of Two Cases
- Diffuse Axonal Injury Associated with Multiple Traumatic Aneurysms of the Distal Anterior Cerebral Artery : Case Report
- Clinical Significance of Traumatic Subarachnoid Hemorrhage
- Nitric oxide contributes to irreversible membrane dysfunction caused by experimental ischemia in rat hippocampal CA1 neurons
- Mild hypothermia protects rat hippocampal CA1 neurons from irreversible membrane dysfunction induced by experimental ischemia
- Short stature as a presenting feature of pheochromocytoma
- Partial resection of the second portion of the duodenum for gastrointestinal stromal tumor after effective transarterial embolization
- Mild Hypothermia Prevents Post-Traumatic Hyperactivity of Excitatory Synapses in Rat Hippocampal CA1 Pyramidal Neurons
- B-41. Experimental Studies on Brain Swelling under Acute Intracranial Hypertension : Especially,sveral factors affecting the Occurrence and Development of Cereberal Vasomotorparesis
- Molecular Cloning and Characterization of a cDNA Encoding the Retinal Arylalkylamine N-Acetyltransferase of the Rainbow Trout, Oncorhynchus mykiss
- Simultaneous Occurrence of Aneurysmal Subarachnoid Hemorrhage and Remote Intracerebral Hemorrhage : Case Report
- The Role of Contrast-Enhanced High Resolution MRI in the surgical Planning of Breast Cancer
- Epithelial differentiation in medulloblastoma : Comparison with other embryonal tumors of neuroectodermal origin
- Acute focal demyelinating disease simulating brain tumors: Histopathologic guidelines for an accurate diagnosis
- Clinicopathological study of pleomorphic xanthoastrocytoma : Correlation between histological features and prognosis
- Pineal malignant rhabdoid tumor with chondroid formation in an adult
- Angiomatous variant of pleomorphic xanthoastrocytoma in a patient with a 20-year history of epilepsy
- Radiation-induced Osteosarcoma of the Calvaria : Case Report
- Multiple Brain Tumors of Different Cell Types with an Unruptured Cerebral Aneurysm : Case Report
- Central Neurocytoma : Case Report
- Postoperative Chronic Subdural Hematoma Following Craniotomy : Four Case Reports
- Clinical Courses and Thyroid Conditions in Three Infants Born to a Mother with Thyroid Stimulating-Blocking Antibodies
- Contralateral Development of Acute Subdural Hematoma Following Surgery for Chronic Subdural Hematoma : Case Report
- Reduction Cranioplasty for Macrocephaly : Two Case Reports
- MOLECULAR CLONING OF cDNA THAT ENCODES THE β SUBUNIT OF TELEOST THYROTROPIN FROM THE GOLDFISH
- Agenesis of the Left Internal Carotid Artery Associated with an Aneurysm of the Anterior Communicating Artery
- MOLECULAR CLONING OF A cDNA THAT ENCODES THE PRECURSOR TO A SALMON-TYPE GONADOTROPIN-RELEASING HORMONE IN THE GOLDFISH
- Microbial Community that Catalyzes Partial Nitrification at Low Oxygen Atmosphere as Revealed by 16S rRNA and amoA Genes(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Completely Thrombosed Giant Fusiform Aneurysm in a Young Patient : Case Report
- Anterior Inferior Cerebellar Artery Dissecting Aneurysm in a Juvenile
- Cervical Myelopathy Due to Compression by Bilateral Vertebral Arteries
- Treatment of Dilute Wastewater in a UASB Reactor at a Moderate Temperature : Microbiological Aspects
- SHP-1 expression in primary central nervous system B-cell lymphomas in immunocompetent patients reflects maturation stage of normal B cell counterparts
- Primary adenocarcinoma of the duodenum demonstrated by ultrasonography
- Na^+/Ca^ exchanger activity induces a slow DC potential after in vitro ischemia in rat hippocampal CA1 region
- Bilateral Anomalous Posterior Inferior Cerebellar Artery-Anterior Inferior Cerebellar Artery Anastomotic Arteries Associated With a Ruptured Cerebral Aneurysm
- Orbital Hemangiopericytoma : Case Report
- Cerebrospinal Fluid following Cerebral Ischemia Accelerates the Proliferation of Bone Marrow Stromal Cells in vitro
- Predictive values of clinical parameters for severe Japanese spotted fever
- Safety Management of a Patient Undergoing Thoracic Aortic Surgery by Spinal Evoked Potential Monitoring
- Treatment of Dilute Wastewater in a UASB Reactor at a Moderate Temperature : Performence Aspects
- Utilization of Dried Pelletized Anaerobic Sludge for Anaerobic Treatment of Wastewater
- Propofol Protects against Anandamide-Induced Injury in Human Umbilical Vein Endothelial Cells
- The tumor of the third ventricle
- Surgical Treatment of Acute Subdural Hematomas in Patients with Low Glasgow Coma Scale Scores
- Effects of Systemic Deep Hypothermia and Subarachnoid Block on the Longitudinally Conducting Evoked Spinal Cord Potentials in Man
- HRAS mutants identified in Costello syndrome patients can induce cellular senescence : possible implications for the pathogenesis of Costello syndrome
- Lightning injury as a blast injury of skull, brain, and visceral lesions : clinical and experimental evidences
- Edaravone Attenuates Impairment of Synaptic Plasticity in Granule Cell Layer of the Dentate Gyrus Following Traumatic Brain Injury
- The Effect of Antineoplaston, a New Antitumor Agent on Malignant Brain Tumors.
- Neuroblastomas of the Central Nervous System. Clinicopathological Features of a Cerebellar Neuroblastoma in Comparison with a Metastatic Cerebral Neuroblastoma.:Clinicopathological Features of a Cerebellar Neuroblastoma in Comparison with a Metastatic Cer
- Transient Global Amnesia and Dural Arteriovenous Fistula of the Anterior Cranial Fossa.
- Traumatic injuries of the cervical cord and spine.
- Decompressive hemicraniectomy for acute subdural hematoma.
- Scanning Electron Microscopic Observations of the Arachnoid Granulations in Monkeys with Cerebrospinal Fluid Hypotension.
- Development of Combined Anaerobic-Aerobic System for Treating Industrial Molasses Wastewater
- The Structure of Ternstroemiaxanthin, a New Aldehydic C40-Carotenoid
- Benign Intracerebellar Cyst without Epithelial Lining.
- The Cystic Growth of a Subependymal Giant-Cell Astrocytoma with Tuberous Sclerosis.
- Prognostic factors associated with mortality in patients undergoing emergency surgery for abdominal aortic aneurysms
- Familial intracranial aneurysms.
- A case of multiple cerebral aneurysms associated with fenestration of the basilar artery.
- Correlation of Histology and Dynamic MR imaging (MRI) of Intracranial Meningiomas with a 0.5 Tesla MR System.
- Brain biopsy for suspected herpes simplex encephalitis. A case report.:A Case Report
- Induction of Neuronal Tolerance by Electroconvulsive Shock in Rats Subjected to Forebrain Ischemia
- Massive cerebellar infarction with obstructive hydrocephalus A case report.:A Case Report
- Traumatic hematoma of the posterior fossa in rare coagulation disorder. Case report.:Case Repor
- Clinicopathological Features of Intraorbital Neurinoma. Report of a Cace.:Report of Two Cases
- Delayed CSF Pseudocyst following Shunt Malfunction after Myelomeningocele Repair.
- Tolosa-Hunt Syndrome with Atypical Intrasellar and Juxtasellal Lesions. Two Case Reports.:Two Case Reports
- Postoperative tension pneumocephalus. Report of 2 cases.:Report of 2 Cases