Monolithic Spin-column Extraction and GC-MS Method for the Assay of Eperisone in Human Serum
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概要
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A rapid gas chromatography-mass spectrometry (GC-MS) method was developed and validated for the analysis of eperisone in serum using monolithic spin-column extraction. The linear concentration range for eperisone was 2-2500 ng/ml. The limit of detection was found to be 0.5 ng/ml. The average extraction recovery range was 92.8-96.0%. The intra- and interday relative standard deviations (RSDs) of the concentrations were less than 12.6% and 12.5%, respectively. The accuracy of this method ranged from 95.0% to 98.3%. We successfully used this assay to analyze serum samples from an eperisone-overdose patient. Our method has some analytical advantages compared with previously reported gas chromatography (GC) and GC-MS methods, such as higher selectivity and sensitivity than GC with nitrogen-phosphorus detection and the avoidance of nonspecificity, the ability to use a smaller sample volume than that required for the GC-MS method, and a shorter sample preparation time than the previous solid-phase extraction (SPE) method.
- 社団法人 日本薬学会の論文
著者
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INOKUCHI Sadaki
Department of Surgery, School of Medicine, Tokai University
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SAITO Takeshi
Department of Surgery, Funabashi Municipal Medical Center
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Yamagiwa Takeshi
Department of Emergency and Critical Care Medicine, Tokai University School of Medicine
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Yui Yuko
GL Sciences Inc.
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Miyazaki Shota
GL Sciences Inc.
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Nakamoto Akihiro
Scientific Investigation Laboratory, Hiroshima Prefectural Police Headquarters
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Namera Akira
Department of Legal Medicine, Graduate School of Biomedical Sciences, Hiroshima University
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Saito Takeshi
Department Of Forensic Medicine Tokai University School Of Medicine
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Inokuchi Sadaki
Department Of Critical And Emergency Medicine Tokai University School Of Medicine
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Nakamoto Akihiro
Scientific Investigation Lab. Hiroshima Prefectural Police Headquarters
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Saito Takeshi
Department Of Cardiovascular Surgery Sendai Open Hospital
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Namera Akira
Department Of Forensic Medicine Graduate School Of Biomedical Sciences Hiroshima University
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Saito Takeshi
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University:The Mushroom Research Institute of Japan:(Present address)General Mushroom Research Center, Japan Applied Microbiology Research Institute, Ltd.
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Saito Takeshi
Department of Emergency and Critical Care Medicine, Tokai University School of Medicine
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Saito Takeshi
Department of Applied Physics, Hokkaido University, Kita-13, Nishi-8, Kita-ku, Sapporo 060, Japan
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