Silencing of Ref-1 Expression by Retrovirus-Mediated shRNA Sensitizes HEK293 Cells to Hydrogen Peroxide-Induced Apoptosis
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概要
- 論文の詳細を見る
Redox factor-1 (Ref-1) is a multifunctional protein involved in DNA base excision repair (BER) and transcription factor modification. By the use of retrovirus-delivered shRNA, we effectively suppressed endogenous Ref-1 expression in human embryonic kidney (HEK) 293 cells. Our results showed that downregulation of Ref-1 rendered cells more sensitive to H2O2-induced apoptosis. In this process, the absence of Ref-1 decreased the ratio of Bcl-2/Bax protein expression, which resulted in cytochrome c release and caspase-3 activation. These data indicate that constitutive Ref-1 is required for cellular defense and that this protective function is dependent on its role in the regulation of Bcl-2 family proteins.
- 社団法人 日本農芸化学会の論文
著者
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ZHANG Yan
Department Molecular Oral Medicine and Maxillofacial Surgery, Division of Frontier Medical Science,
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Zhang Yang
Department of Orthodontics, School of Stomatology, China Medical University
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XIONG Jian-Jun
Department of Biochemistry and Molecular Biology, Sun Yat-sen University
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WANG Jia-Li
Department of Biochemistry and Molecular Biology, Sun Yat-sen University
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BAO Guo-Dong
Department of Biochemistry and Molecular Biology, Sun Yat-sen University
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ZHU Zhen-Yu
Department of Biochemistry and Molecular Biology, Sun Yat-sen University
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