Modified Method for Purifying Rat Liver Branched-Chain α-Ketoacid Dehydrogenase Complex
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概要
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Branched-chain α-ketoacid dehydrogenase complex (BCKDC) is a rate-limiting enzyme in the branched-chain amino acid catabolic pathway. We have developed a method of BCKDC purification from rat liver using hydrophobic interaction column chromatography (Shimomura et al., Arch. Biochem. Biophys., 283, 293–299 (1990)). Here we report a modification of the method designed to obtain the purified enzyme with high reproducibility.
著者
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Bajotto Gustavo
Department of Health Science, Faculty of Psychological and Physical Science, Aichi Gakuin University
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AKITA Keiichi
Department of Materials Science and Engineering, Nagoya Institute of Technology
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Fujimura Yukihiro
Department of Materials Science and Engineering, Nagoya Institute of Technology
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SHIMOMURA Yoshiharu
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Muramatsu Yuji
Department of Materials Science and Engineering, Nagoya Institute of Technology
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