Effect of Enumeration Method on Bifidobacterium Cell Counts in Commercial Powder Products
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概要
- 論文の詳細を見る
In this study, the effects of enumeration methods on viable bifidobacterial counts in powder products were examined to determine the most appropriate enumeration conditions. The bifidobacterial counts of nine commercial powder products containing Bifidobacterium species were determined by a pour plate method using different diluents including Mitsuokas buffer and Ringers solution. Mitsuokas buffer gave significantly higher bifidobacterial counts than Ringers solution when used as diluent for eight of nine commercial powder products containing various Bifidobacterium species. The counts obtained with Ringers solution were on average 51.6% of those obtained using Mitsuokas buffer. Addition of Tween 80 or phosphate buffer to Ringers solution increased the bifidobacterial counts. Also, the diluent had a significantly greater impact in the suspension step than in the subsequent dilution step. In conclusion, the diluent has great effect on the enumeration of bifidobacteria when using the plate count method. Also, the composition of diluents influences bifidobacterial counts.
著者
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YAESHIMA Tomoko
Food Science and Technology Institute, Morinaga Milk Industry Co., Ltd.
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IWATSUKI Keiji
Food Science and Technology Institute, Morinaga Milk Industry Co., Ltd.
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Yaeshima Tomoko
Food Sci. & Technol. Inst. Morinaga Milk Ind. Co. Ltd.
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Iwatsuki Keiji
Food Sci. & Technol. Inst. Morinaga Milk Ind. Co. Ltd.
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Muto Masamichi
Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd.
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Abe Fumiaki
Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd.
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