Enhancing Effects of Sericin on Corneal Wound Healing in Otsuka Long-Evans Tokushima Fatty Rats as a Model of Human Type 2 Diabetes
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概要
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The protein sericin is the main constituent of silk. We investigated the effects of sericin on corneal wound healing in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model for human type 2 diabetes. Corneal wounds were prepared by removal of the corneal epithelium, and documented using a TRC-50X equipped with a digital camera. Sericin solutions were instilled into the eyes of rats five times a day following corneal abrasion. Plasma glucose and triglycerides were determined using an Accutrend GCT. Cholesterol and insulin were measured using a Cholesterol E-Test Kit and ELISA Insulin Kit, respectively. The plasma levels of glucose, triglycerides, cholesterol and insulin in 38-week-old OLETF rats were significantly higher than in Long-Evans Tokushima Otsuka (LETO) rats used as normal controls, and the rate of corneal wound healing in OLETF rats was slower than in LETO rats. The corneal wounds of rats instilled with saline showed almost complete healing by 72 h after corneal epithelial abrasion. On the other hand, the corneal healing rate of OLETF rats instilled with 10% sericin solution was significantly higher than that of LETO rats instilled with saline, and the wounds showed almost complete healing at 48 h after abrasion. The corneal healing rate increased with increasing sericin concentration. The present study demonstrates that the corneal wound healing rate in OLETF rat is slower than in LETO rats, and the instillation of sericin solution has a potent effect in promoting wound healing and wound-size reduction in LETO and OLETF rats.
著者
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Nagai Noriaki
School of Pharmacy, Kinki University
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Murao Takatoshi
School of Pharmacy, Kinki University
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Ito Yoshimasa
School of Pharmacy, Kinki University
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Okamoto Norio
Department of Ophthalmology, Hyogo College of Medicine
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Sasaki Masahiro
Technical Center, Seiren Co., Ltd.
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Okamoto Norio
Department Of Ophthalmology Ntt West Osaka Hospital
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Ito Yoshimasa
School Of Pharmacy Kinki Univ.
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Nagai Noriaki
School Of Pharmacy Kinki Univ.
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Nagai Noriaki
School Of Pharmaceutical Sciences Kindai University
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Ito Yoshimasa
School Of Pharmacy Kindai University:pharmaceutical Research And Technology Institute Kindai Univers
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Ito Yoshimasa
School Of Pharmaceutical Sciences Kinki University
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Nagai Noriaki
School of Pharmaceutical science, Kinki University
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Ito Yoshimasa
School of Pharmaceutical science, Kinki University
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