High-Density Lipoprotein-Associated α-Tocopherol Uptake by Human Retinal Pigment Epithelial Cells (ARPE-19 Cells): the Irrelevance of Scavenger Receptor Class B, Type I
スポンサーリンク
概要
- 論文の詳細を見る
The purpose of this study was to determine the high-density lipoprotein (HDL)-associated α-tocopherol (α-tocopherol-HDL) transport and clarify the contribution of scavenger receptor class B, type I (SR-BI) to the uptake in the human retinal pigment epithelial cell line (ARPE-19 cells). [3H]α-Tocopherol-HDL uptake into ARPE-19 cells seeded onto a transwell from both the apical (apical-to-cell) and basal compartment (basal-to-cell) exhibited a time-dependent increase for 90 min and there was a reduction at 4 °C. These results indicate the involvement of carrier-mediated process in α-tocopherol-HDL uptake in ARPE-19 cells. Immunoblot analysis revealed that SR-BI protein was expressed in ARPE-19 cells. However, the uptake of [3H]α-tocopherol from the apical or basal compartment was hardly inhibited by block lipid transport-1 (BLT-1), a specific inhibitor of the SR-BI-mediated lipid transfer. In conclusion, ARPE-19 cells have a carrier-mediated transport mechanism of [3H]α-tocopherol-HDL regardless of any SR-BI-mediated process.
- 公益社団法人 日本薬学会の論文
著者
-
YAMAMOTO Atsushi
Department of Surgery, Hiroshima Prefectural Akitsu Hospital
-
Tachikawa Masanori
Department Of Pharmaceutics Graduate School Of Medicine And Pharmaceutical Sciences University Of To
-
Hosoya Ken-ichi
Department Of Molecular Biopharmacy And Genetics Graduate School Of Pharmaceutical Sciences Tohoku U
-
Akanuma Shin-ichi
Department Of Pharmaceutics Graduate School Of Medicine And Pharmaceutical Sciences University Of To
-
Akanuma Shin-ichi
Department Of Nuclear Engineering Faculty Of Engineering Kyoto University
-
Okayasu Shun
Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of
-
Yamamoto Atsushi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
-
Yamamoto Atsushi
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University
-
Yamamoto Atsushi
Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
関連論文
- Multicentric Breast Carcinoma: Evaluation of Clinicopathological and Immunohistochemical Characteristics
- The effect of bark decortication for hiwada production on xylem and phloem formation in Chamaecyparis obtusa
- Evaluation of Bile Acids and Fusidate Derivative as Nasal Absorption Enhancers Using an Electrophysiological Technique
- ANTITUMOR AGENT POLY (AMINO ACID) CONJUGATES AS A DRUG CARRIER IN CANCER CHEMOTHERAPY
- Involvement of Reduced Folate Carrier 1 in the inner Blood-Retinal Barrier Transport of Methyltetrahydrofolate
- Molecular Cynamics Simulations of Bovine Cathepsin B and Its Complex with CA074
- Substrate Specificity of Bovine Cathepsin B and Its Inhibition by CA047, Based on Crystal Structure Refinement of the Complex
- Precipitation in an Al-300ppm Fe Alloy
- A Fast and Simple Analysis of Glyphosate in Tea Beverages by Capillary Electrophoresis with On-Line Copper(II)-Glyphosate Complex Formation
- Usefulness of Catechins and Caffeine Profiles to Determine Growing Areas of Green Tea Leaves of a Single Variety, Yabukita, in Japan
- Inhibition of Dehydroascorbic Acid Transport across the Rat Blood-Retinal and -Brain Barriers in Experimental Diabetes(Biopharmacy)
- A Case of Pulmonary Metastasis from Breast Cancer Following an 18-year Disease-Free Interval that Responded to Tamoxifen Treatment
- Generalized Peritonitis with Pneumoperitoneum Caused by the Spontaneous Perforation of Pyometra Without Malignancy: Report of a Case
- K Shell Excitations for Boron and Fluorine by Heavy and Light Ion Bombardments
- Angular Distributions of K-Shell Auger Electrons for Carbon, Nitrogen, Oxygen and Neon Excited by Energetic Ions
- Evaluation of Amino Acid-Mustard Transport as L-Type Amino Acid Transporter 1 (LAT1)-Mediated Alkylating Agents(Biopharmacy)
- A Push-Pull Straining Device for HVEM In-Situ Deformation Experiment
- Properties of 2-Acetyl-7-nonylfluorene and Its O-Analogs in the Alkyl Chain
- Serial Magnetic Besonance Imaging Findings of Rasmussen's Encephalitis : Case Report
- 1P413 Role of Phe-1010 in the light-induced structural changes of LOV2 domain of Adiantum Phytochrome3(17. Light driven system,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- In Situ Laser Microscopy on Corrosion in Deposition Coated Magnesium Alloy
- Deposition Coating of Magnesium Alloys with Pure Magnesium
- Convergent Beam Dark Field Imaging Technique with Its Application to Observation of Multiphase Materials
- Mycobacterium abscessus Corneal Ulcer Following Sutured Clear Corneal Cataract Incision
- Hypertonicity Enhances GABA Uptake by Cultured Rat Retinal Capillary Endothelial Cells
- HPLC / fluorescence detected circular dichroism detection by three-dimensional fluorescence detector
- D-Serine Dehydratase from Chicken Kidney : A Vertebral Homologue of the Cryptic Enzyme from Burkholderia cepacia
- Functional and Structural Characterization of D-Aspartate Oxidase from Porcine Kidney : Non-Michaelis Kinetics due to Substrate Activation
- Clinical and experimental studies of quantitative ultrasound measurement in the os calcis : Prevalence and incidence of vertebral fracture
- Changes in Microstructures during Annealing after Cold-Rolling at 30% and 50% Reduction on Al-Mg-Si Alloy
- Chiral Capillary Electrophoresis of Agrochemicals in Real Samples
- Microscopic Theory of the CDW State of 1T-TiSe_2
- Enzymatic Reduction of cystine into Cysteine by Cell-Free Extract of Clostridium thermoaceticum
- High-Density Lipoprotein-Associated α-Tocopherol Uptake by Human Retinal Pigment Epithelial Cells (ARPE-19 Cells): the Irrelevance of Scavenger Receptor Class B, Type I
- Conditionally Immortalized Cell Lines as a New In Vitro Model for the Study of Barrier Functions
- Vortex-Induced Topological Transition of the Bilinear-Biquadratic Heisenberg Antiferromagnet on the Triangular Lattice(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Roles of organic anion/cation transporters at the blood-brain and blood-cerebrospinal fluid barriers involving uremic toxins
- Lower Cretaceous fresh-water stromatolites from northern Kyushu, Japan
- Prevalence of de Quervain’s Disease in the General Population and Risk Factors
- Nonadiabatic Dynamics of Ultracold Fermions in Optical Superlattices
- $Z_{2}$-Vortex Ordering of the Triangular-Lattice Heisenberg Antiferromagnet
- Characteristics of Cortical Activation in Schizophrenia during the Card Game "Concentration"
- Trapped Ultracold Fermions in Double-Well Optical Lattices