Suppression of N-Myc Downstream-Regulated Gene 2 Is Associated with Induction of Myc in Colorectal Cancer and Correlates Closely with Differentiation
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概要
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NDRG2, a new member of the N-Myc downstream-regulated gene (NDRG) family, is a focus for study at present. Up to now, its expression and function in carcinoma remain to be elucidated. In this study, using a colorectal cancer tissue array and a series of 213 colorectal cancer samples, the relationship between Ndrg2 and c-MYC expression and tumor differentiation level was investigated. Immunohistochemistry showed that Ndrg2 expression was reduced and that c-Myc was increased in colorectal carcinomas. In addition, Ndrg2 protein levels increased from poorly differentiated to well-differentiated carcinomas (p=0.005). Real-time polymerase chain reaction and Western blots demonstrated quantitatively that NDRG2 mRNA and protein levels were lower in colorectal carcinomas compared to the adjacent tissue and normal tissue from the same individual (p=3×10−8). Also, the NDRG2 expression level in adjacent carcinoma tissue was lower than that of normal tissue. However, the expression pattern of c-MYC was the inverse (p=5×10−8). Finally, we induced the differentiation of the colorectal carcinoma cell lines HT29, SW480 and SW620 and found that NDRG2 expression increased and that c-MYC expression declined with increasing differentiation. These novel data show a disparity in both the mRNA and protein expression levels of Ndrg2 and c-Myc between colorectal cancers and normal tissues. Taken together, NDRG2 may play a role during the differentiation of colorectal cancer cells, and the function of NDRG2 in the development of colorectal cancer should be further investigated.
- 公益社団法人 日本薬学会の論文
著者
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Shi Hai
State Key Laboratory Of Cancer Biology Department Of Gastrointestinal Surgery Xijing Hospital The Fo
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Shi Hai
Department Of Electronic Engineering City University Of Hong Kong
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Zhang Jian
Department Of Cardiology Rui Jin Hospital Shanghai Jiao Tong University School Of Medicine
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YAO Libo
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fourth Military Medical
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Chen Changsheng
Department Of Fisheries Oceanography School For Marine Science And Technology University Of Massachu
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Jin Haifeng
State Key Laboratory Of Cancer Biology Department Of Gastrointestinal Surgery Xijing Hospital The Fo
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Fan Daiming
State Key Laboratory Of Cancer Biology And Xijing Hospital Of Digestive Diseases Fourth Military Med
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Wang Weizhong
Department Of Cardiology Dhmc University Of Colorado Health Sciences Center
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Chu Dake
Department of Gastrointestinal Surgery, Xijing Hospital, The Fourth Military Medical University
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Xu Chunsheng
Department of Gastrointestinal Surgery, Xijing Hospital, The Fourth Military Medical University
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Zhang Jian
Department Of Biology Huaihua College
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Jin Haifeng
State Key Laboratory of Cancer Biology & Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University
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Shi Hai
Department of Biochemistry and Molecular Biology, The State Key Laboratory of Cancer Biology, The Fourth Military Medical University
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Wang Weizhong
Department of Gastrointestinal Surgery, Xijing Hospital, The Fourth Military Medical University
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Yao Libo
Department of Biochemistry and Molecular Biology, The State Key Laboratory of Cancer Biology, The Fourth Military Medical University
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Fan Daiming
State Key Laboratory of Cancer Biology & Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University
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