羊腱板由来細胞に対するTGF-β1投与が各種遺伝子発現に与える影響

概要

論文の詳細を見る
Although good mid-term clinical results after a rotator cuff repair have been reported on, it was pointed out that complete tendon reattachment to the humerus is very difficult. The aim of the present study was to investigate the effect of TGF-β1 on a sheep infraspinatus tendon cell cultured on Polyglactin910. The cells derived from a sheep infraspinatus tendon by an enzyme digestion method were cultured for 10 days.After the culture, expression of genes were investigated by RT-PCR methods after the extraction of RNA with TRIzole treatment. Evaluated genes were type 1 and 3 collagen, MMP-1 and MMP-13, Aggrecan, Biglycan, Decorin, Fibromodulin, Lumican, Elastin, Osteopontin. Statical comparisons were performed using one-way analysis of variance and Fisher PLSD tests. Differences were considered significant for less than 5%. In results, mRNA expression was significantly increased for type 1 collagen, Aggrecan, Elastin. Especially, type 1 collagen, Elastin were revealed a dose dependent positive effect. mRNA expression was, however, significantly decreased for MMP-1, Lumican.These results indicated that TGF-β1 may have a significant effect on rotator cuff healing and stimulus for further studies in this field.
日本肩関節学会の論文