Initial bFGF Distribution Affects the Depth of Three-dimensional Microvessel Networks in Vitro
スポンサーリンク
概要
- 論文の詳細を見る
Control of three-dimensional (3D) microvessel formation is critical for regenerative medicine and tissue engineering because vessels are essential for the formation and maintenance of organ function. In order to function, tissues need an internal network of vessels. To introduce a 3D vessel network deep into the tissue, it is necessary to introduce 3D microvessel control which makes it a critical factor in regenerative medicine and tissue engineering. This study focuses on the effect of the concentration gradient of growth factors used in seeding the endothelial cells (ECs) on the morphology of the network. First, ECs were seeded using two model environments: collagen gel containing bFGF and incubated without bFGF medium (gel-bFGF model), and collagen gel containing no bFGF and incubated with bFGF medium (medium-bFGF model). The networks were observed in 3D with confocal laser scanning microscopy. The migration of ECs on the collagen gel was analyzed to study the effect of the concentration gradient on the network formation process. We found that the ECs of the gel-bFGF model showed significantly longer migration distance and more sprouting points compared with those of the medium-bFGF model. The networks of the gel-bFGF model, expanded mainly in a depth of 20-30 μm, and many reached a depth of 50-60 μm, whereas many networks in the medium-bFGF model expanded in a depth of only 10-20 μm. These results revealed that the initial growth factor distribution affects (a) both EC migration of the network formation process and the number of sprouting points, and (b) network morphology.
- 一般社団法人 日本機械学会の論文
著者
-
Ikeda Mariko
Keio Leading-edge Laboratory Of Science And Technology Keio University
-
Sudo Ryo
School of Fundamental Science and Technology, Graduate School of Keio University
-
Tanishita Kazuo
School Of Allied Health Sciences Kitasato University
-
Kudo Susumu
Department of Engineering, Shibaura Institute of Technology
-
UEDA Akinori
School of Fundamental Science and Technology, Keio University
-
IKEDA Mariko
Keio Leading-edge Laboratory of Science and Technology, Keio University
関連論文
- P-17 DESIGN OF MULTILAYERED COCULTURE SYSTEM COMPOSED OF SMALL HEPATOCYTES, LIVER STELLATE CELLS AND SINUSOIDAL ENDOTHELIAL CELLS
- Albumin Permeability across Endothelial Monolayers under Long-Term Shear Stress(Bioengineering)
- Effect of Fluid Force on Vascular Cell Function
- Relationship between Microtubule Network Structure and Intracellular Transport in Cultured Endothelial Cells Affected by Shear Stress(Bioengineering)
- Effect of Shear Stress on the Formation of Three-Dimensional Angiogenesis Network(Micro- and Nano-biomechanics)
- Reconstruction of Ductular Structure by Rat Bile Ductular Epithelial Cells(Micro- and Nano-biomechanics)
- Effect of Long-Term Shear Stress Exposure on Calcium Response and Morphology of Cultured Endothelial Cells(Bioengineering)
- Techniques of SNP Genotyping and Remote Sensing Applied for Elucidation of the Contribution of Polygene and Environmental Factors to Inter-individual Variation in Skin Pigmentation Phenotype
- 2-1 Effect of Fluid Frictional Force on ATP Synthesis of Vascular Cell
- 2-18 Vascular Cell Coordination Induced by Blood Flow(Proceedings of the 53rd Meeting of Japan Society of Physiological Anthropology)
- S10-1 Effect of Low Temperature on Endothelial Cell Nitric Oxide Synthase Activity(Proceedings of The 8th International Congress of Physiological Anthropology)
- 1-4 Molecular-biological Study on Regulatory Mechanism of Cutaneous Blood Flow(Proceedings of the 55th Meeting of Japan Society of Physiological Anthropology)
- Effect of stent placement on flow structure in the realistic cerebral aneurysm model(3D3 Biorheology & Microcirculation II)
- A-37. Significance of Specialized Intercellular Junctions in Human Intracrainal Germinoma
- A-59. Cerebral Artery Replacement by Use of High Voltage Cathode Ray Irradiated Homologous Artery A study of static dynamics
- 2-1 Effect of Fluid Frictional Force on Vascular Cell Function(Proceedings of the 51st Meeting of Japan Society of Physiological Anthropology)
- P-5 Effect of Blood Flow on Calcium Accumulation in Artery(Proceedings of the 53rd Meeting of Japan Society of Physiological Anthropology)
- Using the Effective Thermal Conductivity to Evaluate the Freezing Process in Medaka Embryos Suspension
- Growth of Endothelial Cells on Surfaces Modified by a Plasma-Polymerized Coating(Organic Molecular Electronics)
- The Stabilization Effect of Mesenchymal Stem Cells on the Formation of Microvascular Networks in a Microfluidic Device
- Initial bFGF Distribution Affects the Depth of Three-dimensional Microvessel Networks in Vitro