Characterization of the S-RNase promoters from sweet cherry (Prunus avium L.)

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概要

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• Genomic DNA fragments containing the S3-, S4-, and S6-RNase genes were isolated from the sweet cherry (Prunus avium L.) and sequenced. Comparison of the 5-flanking sequences of these three S-RNases indicated that a highly conserved region (designated CR) existed just upstream from the putative TATA boxes. We postulate that CR contains cis-regulatory element(s) involved in pistil expression. To examine the activity of the isolated S-RNase promoters of sweet cherry in the pistil, we transiently introduced approximately 650-bp fragments of the S4- and S6-RNase promoters fused to β-glucuronidase (GUS) gene into the pistil of the petunia using a particle bombardment technique. Histochemical analysis showed that the 5-flanking region of each S-RNase was active in the pistil. This suggests that cis-regulatory element(s) for pistil-specific expression may exist(s) within the 650-bp region upstream from the TATA box in the sweet cherry S-RNase promoter.

• 日本遺伝学会の論文
• 2003-04-01

著者

• SUZUKI Takashi

  Faculty of Science,Hiroshima University

• Nakano Hideaki

  Yamagata Prefecture Agricultural Experiment Station

• Kitashiba Hiroyasu

  Jst Regional Joint Research Project Of Yamagata Prefecture

• ISHIZAKA Takako

  JST, Regional Joint Research Project of Yamagata Prefecture

• KITASHIBA Hiroyasu

  JST, Regional Joint Research Project of Yamagata Prefecture

• Ishizaka Takako

  Jst Regional Joint Research Project Of Yamagata Prefecture

• Suzuki T

  Graduate Univ. For Advanced Studies (sokendai) Hayama Jpn

• Suzuki Takashi

  Faculty Of Advanced Techno-surgery Institute Of Advanced Biomedical Engineering And Science Tokyo Wo

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