Cloning and characterization of two full-length cDNAs, TaGA1 and TaGA2, encoding G-protein α subunits expressed differentially in wheat genome
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概要
- 論文の詳細を見る
In the present study, we identified and characterized two cDNAs, named TaGA1 and TaGA2, encoding α subunits of heterotrimeric G proteins synthesized from one-week-old seedling mRNAs of common wheat cv. S615 using RACE PCR and RT-PCR methods. The clone TaGA1 contained an open reading frame that encoded a protein consisting of 383 amino acid residues with a molecular mass of 51.3 kDa, whereas the clone TaGA2 contained an open reading frame encoding 390 amino acids with a molecular mass of 52.5 kDa. At the amino acid level, both cDNAs (TaGA1 and TaGA2) showed 70-96% and 30-40% homologies to plant and animal G-protein alpha (G α) subunits, respectively, and 97.7% homology to each other. The regions essential for binding to GTP were conserved among all G α subunits in higher plants and mammals examined. However, the C-terminal amino acid sequences of TaGA1 and TaGA2 were similar to those of cereal G α subunits (rice and barley) but were different from the analogous sequences of mammalian G α subunits as well as from those of the leguminous and Solanaeceous G α subunits. Southern analysis revealed that the hexaploid wheat genome contained three major copies of G α subunit gene with a few less homologous copies. The analysis of the expression for G α subunit genes in wheat showed that both TaGA1 and TaGA2 mRNAs were abundant in one-week-old seedlings, immature seeds harvested one-week after anthesis, young spikes and internodes, indicating constitutive expression patterns in all of the organs tested. Especially, young spikes and internodes exhibited increased levels of mRNA accumulation, suggesting that G α subunit gene is highly expressed in actively elongating and fast growing tissues. Moreover, both TaGA1 and TaGA2 showed genome-specific expressions in wheat and may participate in the light-regulated growth and development of the seedlings.
- 日本遺伝学会の論文
- 2003-04-01
著者
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Harada Kyuya
Laboratory Of Genetics And Plant Breeding Faculty Of Horticulture Graduate School Of Science And Tec
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Koba Takato
Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University
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Koba Takato
Laboratory Of Genetics And Plant Breeding Faculty Of Horticulture Graduate School Of Science And Tec
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Koba Takato
Laboratory Of Genetics And Plant Breeding Faculty Of Horticulture Chiba University
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HOSSAIN Shakhawat
Laboratory of Genetics and Plant Breeding, Graduate School of Science and Technology, Chiba Universi
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Hossain Shakhawat
Laboratory Of Genetics And Plant Breeding Faculty Of Horticulture Graduate School Of Science And Tec
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Hossain Md.
Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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