Cloning and Characterization of a cDNA (TaGB1) Encoding β Subunit of Heterotrimeric G-protein from Common Wheat cv. S615
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概要
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We isolated a full-length cDNA clone, TaGB1, encoding the β subunit of heterotrimeric G-protein from common wheat, Triticum aestivum cv. S615. The predicted amino acid sequence deduced from the cDNA was 70-89% and >50% homologous to those of the β subunits of plants and animals, respectively. The TaGB1 exhibited the higest homology of 89% with the rice G-protein β subunit gene and preserved the most essential seven repeats of the “WD-40” motif, which are commonly found in all plant and animal G-protein β subunits. Southern hybridization revealed that the wheat genome contains a single-copy gene for the β subunit of heterotrimeric G-protein. The analysis of the expression for Gβ in wheat showed that TaGB1 mRNA was expressed in all of the organs tested and exhibited high degrees of mRNA accumulation in spikes and internodes as well as dark-grown seedlings. It was also observed by RT-PCR that the transcript of TaGB1 was expressed throughout the ancestral genomes of wheat.
- 日本植物細胞分子生物学会の論文
- 2003-06-01
著者
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Harada Kyuya
Laboratory Of Genetics And Plant Breeding Faculty Of Horticulture Graduate School Of Science And Tec
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Koba Takato
Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University
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Koba Takato
Laboratory Of Genetics And Plant Breeding Faculty Of Horticulture Chiba University
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Koba Takato
Laboratory Of Genetics And Plant Breeding Graduate School Of Science And Technology Chiba University
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HOSSAIN Shakhawat
Laboratory of Genetics and Plant Breeding, Graduate School of Science and Technology, Chiba Universi
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Hossain Shakhawat
Laboratory Of Genetics And Plant Breeding Faculty Of Horticulture Graduate School Of Science And Tec
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Hossain Shakhawat
Laboratory Of Genetics And Plant Breeding Graduate School Of Science And Technology Chiba University
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Harada Kyuya
Laboratory Of Genetics And Plant Breeding Graduate School Of Science And Technology Chiba University
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Koba Takako
Laboratory of Genetics and Plant Breeding, Graduate School of Science and Technology, Chiba University
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