Analyis sof homology of small plasmid-like mitochondrial DNAs in the diferent cytoplasmic male sterile strains in rice.
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概要
- 論文の詳細を見る
Mitochondrial DNAs (mtDNAs) were isolated from several rice strains of Chinsurah boro II (fertile), Zhen Shan 97A (male sterile), Zhen Shan 97 (fertile), and Akihikari (normal japonica). Small plasmid-like DNAs, B1 and B2, which were found firstly in BT line, were cloned into plasmid vectors and mapped in terms of some of restriction enzymes. Plasmid-like DNAs were also observed in the mitochondria of Zhen Shan 97A and Zhen Shan 97 by agarose gel electrophoresis. To examine whether these plasmids were identical or not, hybridization analyses were carried out with B1 and B2 as probes. Consequently, it was suggested that two plasmids of Zhen Shan 97A were identical to B1 and B2, and one of the plasmids of Zhen Shan 97 was identical to B2. The smaller plasmid of Zhen Shan 97 had no homology to either B1 or B2. Co-existence of B1 and B2, while common, is not an obligatory feature. Because B2 was found in the fertile cytoplasm of Zhen Shan 97, an involvement of B2 with cytoplasmic male sterility (cms) might be denied.
- 日本遺伝学会の論文
著者
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Tano Shigemitsu
Laboratory Of Radiation Genetics And Chemical Mutagenesis Faculty Of Agriculture The University Of T
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YAMAGUCHI HIKOYUKI
Laboratories of Radiation Genetics and Biometrics, University of Tokyo
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Tsutsumi Nobuhiro
Laboratory Of Plant Molecular Genetics Graduate School Of Agricultural And Life Sciences The Univers
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Sakamoto Wataru
Laboratory Of Fisheries And Environmental Oceanography Graduate School Of Agriculture Kyoto Universi
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TANO Shigemitsu
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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TSUTSUMI Nobuhiro
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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SAKAMOTO Wataru
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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MOMOSE Masaki
Plant Laboratory, Kirin Brewery Co.
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YAMAGUCHI Hikoyuki
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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