The gene for alternative oxidase-2(AOX2) from Arabidopsis thaliana consists of five exons unlike other AOX genes and is transcribed at an early stage during germination
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概要
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We investigated the expressions of genes for alternative oxidase (AOX1a, AOX1b, AOX1c and AOX2) and genes for cytochrome c oxidase (COX5b and COX6b) during germination of Arabidopsis thaliana, and examined oxygen uptakes of the alternative respiration and the cytochrome respiration in imbided Arabidopsis seeds. A Northern blot analysis showed that AOX2 mRNA has already accumulated in dry seeds and subsequently decreased, whereas accumulation of AOX1a mRNA was less abundant from 0 hours to 48 hours after imbibition and then increased. The increase of the capacity of the alternative pathway appeared to be dependent on the expressions of both AOX2 and AOX1a. On the other hand, steady-state mRNA levels of COX5b and COX6b were gradually increased during germination, and the capacity of the cytochrome pathway was correlated with the increase of expressions of the COX genes. Antimycin A, the respiratory inhibitor, strongly increased the expression of AOX1a but had no effect on the expression of AOX2. A 5’RACE analysis showed that AOX2 consists of five exons, which is different from the case of most AOX genes identified so far. Analysis of subcellular localization of AOX2 using green fluorescent protein indicated that the AOX2 protein is imported into the mitochondria.
- 日本遺伝学会の論文
著者
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Tsutsumi Nobuhiro
Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The Unive
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Nakazono Mikio
Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The Unive
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HIRAI Atsushi
Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The Unive
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Kwang -
Laboratory Of Crop Ecology And Morphology School Of Agrigultural And Life Sciences The University Of
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Hirai A
School Of Agriculture Meijo University
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Nakazono M
Laboratory Of Plant Molecular Genetics Graduate School Of Agricultural And Life Sciences The Univers
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Saisho Daisuke
Research Institute For Bioresources Okayama University
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Saisho Daisuke
Research Institute For Bioresources (rib) Okayama University
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Akita Shigemi
Laboratory of Crop Ecology and Morphology, Faculty of Agriculture, The University of Tokyo
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Tsutsumi N
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Tsutsumi Nobuhiro
Laboratory Of Plant Molecular Genetics Graduate School Of Agricultural And Life Sciences The Univers
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Akita Shigemi
Laboratory Of Crop Ecology And Morphology Faculty Of Agriculture The University Of Tokyo
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Akita Shigemi
Laboratory Of Crop Ecology And Morphology School Of Agrigultural And Life Sciences The University Of
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Lee Kwang-Hong
Laboratory of Crop Ecology and Morphology, School of Agrigultural and Life Sciences, The University of Tokyo
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Saisho Daisuke
Research Institute for Bioresources, Okayama University
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