アンチセンスオリゴヌクレオチドによるオオムギアリュ-ロン層におけるα-アミラ-ゼ遺伝子発現の抑制〔英文〕
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概要
- 論文の詳細を見る
Antisense oligodeoxynucleotides (ODNs) have been applied to regulate gene expression using cell-free media or animal cells. Here we demonstrate the specific inhibition of barley α-amylase gene expression by synthetic antisense ODNs. In a cell free system using wheat-germ extracts, 5 μM of a 20-mer antisense ODN prevented the synthesis of the polypeptide corresponding to the predetermined length of α-amylase translated in vitro, whereas there was no effect on other protein synthesis. Furthermore, in cultured aleurone cells, a-amylase activity was efficiently decreased by addition of ODNs. At the concentrations higher than 5 μM, antisense ODN inhibited α-amylase gene expression almost completely. These results imply that ODN could transport into the cultured aleurone cells crossing the cell membrane, and regulate specific gene expression. This simple model system could be applicable not only for the analysis of the α-amylase multigene family in barley but also for studying functions of cryptic genes in higher plant.
- 日本遺伝学会の論文
- 1992-04-25
著者
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堤 伸浩
Laboratory of Radiation Genetics, Faculty of Agriculture, The University of Tokyo
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Tano Shigemitsu
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, the University of
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Tano Shigemitsu
Laboratory Of Radiation Genetics And Chemical Mutagenesis Faculty Of Agriculture The University Of T
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Tano Shigemitsu
Laboratory Of Radiation Genetics And Chemical Mutagenesis Faculty Of Agriculture University Of Tokyo
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TSUTSUMI Nobuhiko
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tok
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KANAYAMA Keiko
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tok
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Kanayama Keiko
Laboratory Of Radiation Genetics And Chemical Mutagenesis Faculty Of Agriculture University Of Tokyo
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Tsutsumi N
Laboratory Of Radiation Genetics And Chemical Mutagenesis Faculty Of Agriculture University Of Tokyo
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田野 茂光
Laboratory of Radiation Genetics, Faculty of Agriculture, The University of Tokyo
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金山 桂子
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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