Successful Mouse Cloning of an Outbred Strain by Trichostatin A Treatment after Somatic Nuclear Transfer
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概要
- 論文の詳細を見る
Although the somatic cloning technique has been used for numerous applications and basic research of reprogramming in various species, extremely low success rates have plagued this technique for a decade. Further in mice, the "clonable" strains have been limited to mainly hybrid F1 strains such as B6D2F1. Recently, we established a new efficient cloning technique using trichostatin A (TSA) which leads to a 2-5 fold increase in success rates for mouse cloning of B6D2F1 cumulus cells. To further test the validity of this TSA cloning technique, we tried to clone the adult ICR mouse, an outbred strain, which has never been directly cloned before. Only when TSA was used did we obtain both male and female cloned mice from cumulus and fibroblast cells of adult ICR mice with 4-5% success rates, which is comparable to 5-7% of B6D2F1. Thus, the TSA treatment is the first cloning technique to allow us to successfully clone outbred mice, demonstrating that this technique not only improves the success rates of cloning from hybrid strains, but also enables mouse cloning from normally "unclonable" strains.
- 日本繁殖生物学会の論文
著者
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Bui Hong-thuy
韓国
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WAKAYAMA Teruhiko
Center for Developmental Biology, RIKEN Kobe
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Wakayama Teruhiko
Center For Developmental Biology Riken Kobe
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Kishigami Satoshi
Department Of Genetic Engineering Kinki University
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Kishigami Satoshi
Center For Developmental Biology Riken Kobe
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SATA Tetsutaro
Department of Pathology, National Institute of Infectious Diseases
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Bui Hong-Thuy
Center for Developmental Biology, RIKEN Kobe
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Wakayama Sayaka
Center for Developmental Biology, RIKEN Kobe
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TOKUNAGA Kenzo
Department of Pathology, National Institute of Infectious Diseases
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THUAN Nguyen
Center for Developmental Biology, RIKEN Kobe
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HIKICHI Takafusa
Center for Developmental Biology, RIKEN Kobe
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MIZUTANI Eiji
Center for Developmental Biology, RIKEN Kobe
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OHTA Hiroshi
Center for Developmental Biology, RIKEN Kobe
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SUETSUGU Rinako
Center for Developmental Biology, RIKEN Kobe
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Sata Tetsutaro
Department Of Pathology Aids Research Center National Institute Of Infectious Diseases
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Tokunaga Kenzo
Department Of Pathology National Institute Of Infectious Diseases
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Wakayama Sayaka
Center For Developmental Biology Riken Kobe
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Hikichi Takafusa
Center For Developmental Biology Riken Kobe
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