Predominant Culturable Bacillus Species in Japanese Arable Soils and Their Potential as Biocontrol Agents
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概要
- 論文の詳細を見る
To reveal predominant culturable Bacillus species in Japanese arable soils, aerobic spore-forming bacteria were isolated from different soils in Japan and their phylogenic positions were estimated from the 16S rDNA sequences. Bacillus species were also isolated from tomato roots grown in soils for 4 weeks to estimate the difference in phylogenic positions between soil and root isolates. The number of culturable Bacillus species ranged from 6.4 to 7.4 log cfu g^<-1> soil and 4.7 to 6.7 log cfu g^<-1> root in soils and tomato roots, respectively, and the proportion of Bacillus species among total culturable bacteria ranged from 3.8% to 17.9% and 0.1% to 6.7% in soils and roots, respectively. The differences in the number and percentage of culturable Bacillus species between the soils and roots were highly significant (P=0.0000 and 0.0023, respectively), indicating that Bacillus species were more predominant in soil than in root. A total of 108 isolates were characterized using the restriction fragment length polymorphism (RFLP) method and partial 16S rDNA sequences. They affiliated to 22 species including the genus Bacillus, Brevibacillus and Paenibacillus. No nitrogen fixers were isolated. The predominant species was Bacillus megaterium to which 42 isolates (39%) belonged. Some of the species, such as Bacillus luciferensis and Brevibacillus agri, were isolated only from the tomato roots and considered to be more adapted to the rhizosphere than soil. Among 108 isolates, only one strain, N2S6, belonging to Bacillus megaterium, showed consistent antagonistic activity against the bacterial wilt of tomato caused by Ralstonia solanacearum.
- 日本微生物生態学会の論文
著者
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TOYOTA KOKI
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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Mochizuki Masami
Idemitsu Kosan Co. Ltd.
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NISHIJIMA TAKANORI
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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Toyota Koki
Graduate School Of Bio-application And System Engineering Tokyo University Agriculture And Technolog
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Toyota Koki
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Nishijima Takanori
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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