Quantification of Pratylenchus penetrans in radish fields using a combination method of soil compaction and real-time PCR to determine the economic threshold(Soil Biology)
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概要
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The objective of this study was to compare the economic threshold (ET) of Pratylenchus penetrans in radish fields of andosols using the Baermann method and the combination method of soil compaction and real-time polymerase chain reaction (PCR). Soil samples were collected from 26 plots at depths of 0 to 30 and 30 to 60cm before seeding of radishes. The number of P. penetrans in each sample was estimated by the Baermann method and the combination method. No P. penetrans was detected in 13 plots by the Baermann method, while the number of plots in which no P. penetrans was detected was only two by the combination method. The number of spots caused by P. penetrans on radishes harvested from the plots was also counted. It was difficult to determine the ET of P. penetrans estimated by the Baermann method, based on whether severe damage (more than 10 spots on average per radish) was seen. However, the ET of P. penetrans estimated by the combination method was determined at 5.3 J2 equivalents per 20g dry soil. In plots with P. penetrans densities lower than the ET as evaluated by the combination method, the ratio of plots with no damage was 87%. The results suggest that the combination method has an advantage in the estimation of damage to radish by nematode.
- 一般社団法人日本土壌肥料学会の論文
著者
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Toyota Koki
Tokyo Univ. Agriculture And Technol. Tokyo
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SUGA YUKO
National Agricultural Research Center for Western Region
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Suga Yuko
National Agricultural Res. Center For Western Region Kyoto Jpn
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Takada Atsushi
Kanagawa Agricultural Technology Center
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Toyota Koki
Graduate School Of Bio-applications And Systems Engineering Tokyo University Agriculture And Technol
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Sato Erika
Tokyo Univ. Agriculture And Technol. Tokyo
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Sato Erika
Graduate School Of Bio-applications And Systems Engineering Tokyo University Agriculture And Technol
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Toyota Koki
Graduate School Of Bio-application And System Engineering Tokyo University Agriculture And Technolog
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Takeuchi Koji
Tokyo Development Foundation For Agriculture Forestry And Fisheries
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SATO Erika
National Agricultural Research Center for Western Region
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KISAKI Chihiro
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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MIYAKE Kazuto
Kanagawa Agricultural Technology Center
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MATSUURA Rie
Tokyo Development Foundation for Agriculture, Forestry and Fisheries
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Matsuura Rie
Tokyo Development Foundation For Agriculture Forestry And Fisheries
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Kisaki Chihiro
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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