Development of a real-time PCR method for the potato-cyst nematode Globodera rostochiensis and the root-knot nematode Meloidogyne incognita(Soil Biology)
スポンサーリンク
概要
- 論文の詳細を見る
The primers PCN280f and NEPCN398r were designed for the quantitative detection of the potato-cyst nematode Globodera rostochiensis using real-time polymerase chain reaction (PCR). One, five, 50, 125 and 250 individuals of the second-stage juveniles (J2) of G. rostochiensis were mixed with various stages of vermiform Caenorhabditis elegans to make a total of 500 individuals and DNA was extracted from the nematode mixture. There was a significant correlation (r^2=0.9355, P<0.001) between the threshold cycle values and the number of G. rostochiensis added. When nematodes were extracted from soils artificially infested with G. rostochiensis to various degrees and real-time PCR was conducted using DNA templates from the nematodes extracted, there was a highly significant correlation in the numbers of G. rostochiensis J2 from the real-time PCR method and morphological identification. Real-time PCR sensitively detected a single G. rostochiensis J2 out of 1,000 individuals of free-living nematodes. Similarly, real-time PCR primers RKNf and RKNr were designed for the detection of the root-knot nematode Meloidogyne incognita. This study demonstrated that the real-time PCR assay for the potato-cyst nematode and the root-knot nematode provides a sensitive and reliable means for the rapid quantification of these vermiform pests.
著者
-
Wada Satoko
Graduate School Of Bio-application And System Engineering Tokyo University Agriculture And Technolog
-
Min Yu
Graduate School Of Bio-application And System Engineering Tokyo University Agriculture And Technolog
-
Sato Erika
Tokyo Univ. Agriculture And Technol. Tokyo
-
Sato Erika
Graduate School Of Bio-applications And Systems Engineering Tokyo University Agriculture And Technol
-
Toyota Koki
Graduate School Of Bio-application And System Engineering Tokyo University Agriculture And Technolog
-
SHIRAKASHI Tomoaki
Graduate School of Bio-Application and System Engineering, Tokyo University Agriculture and Technolo
-
Shirakashi Tomoaki
Graduate School Of Bio-application And System Engineering Tokyo University Agriculture And Technolog
関連論文
- Suppression of Bacterial Wilt and Fusarium Wilt by a Burkholderia nodosa Strain Isolated from Kalimantan Soils, Indonesia
- Suppression of ammonia volatilization from a paddy soil fertilized with anaerobically digested cattle slurry by wood vinegar application and floodwater management(Environment)
- Soil microbial biomass phosphorus as an indicator of phosphorus availability in a Gleyic Andosol(Soil Biology)
- Suppression of Bacterial Wilt of Tomato by Ralstonia solanacearum by Incorporation of Composts in Soil and Possible Mechanisms
- Development of a real-time PCR method for the potato-cyst nematode Globodera rostochiensis and the root-knot nematode Meloidogyne incognita(Soil Biology)
- Enhancement of Nitrogen-fixing Activity of Enterobacteriaceae Strains Isolated from Sago Palm (Metroxylon sagu) by Microbial Interaction with Non-nitrogen Fixers
- Relationships between the damage to radish caused by the root-lesion nematode Pratylenchus penetrans, its density prior to cultivation and the soil nematode community structure evaluated by polymerase chain reaction-denaturing gradient gel electrophoresis
- Effect of different organic amendments on the resistance and resilience of the organic matter decomposing ability of soil and the role of aggregated soil structure(Soil Biology)
- Phylogenetic distribution of bacteria isolated from the floodwater of a Japanese paddy field(Soil Chemistry and Mineralogy)
- Functional Characterization of Soil Microbial Communities Based on the Utilization Pattern of Aromatic Compounds (Soil Biology)
- Effect of Moisture Conditions and Pre-Incubation at Low Temperature on Bacterial Wilt of Tomato Caused by Ralstonia solanacearum
- Comparison of Phospholipid Fatty Acid Composition in Floodwater and Plow Layer Soil during the Rice Cultivation Period in a Japanese Paddy Field
- Seasonal Variations of Phospholipid Fatty Acid Composition in the Floodwater of a Japanese Paddy Field under a Long-Term Fertilizer Trial
- Predominant Culturable Bacillus Species in Japanese Arable Soils and Their Potential as Biocontrol Agents
- Susceptibility of soils with or without repeated application of farmyard manure to bacterial wilt of tomato caused by Ralstonia solanacearum
- Tin Concentrations in River / Bay Sediments of Tokyo in 1984 and 2000
- Comparison of Disease Suppressiveness of Different Soils with or without Repeated Application of Organic Matters toward Bacterial Wilt of Tomato Caused by Ralstonia solanacearum
- Effect of Three Organophosphorous Nematicides on Non-target Nematodes and Soil Microbial Community
- Mechanism of Tomato Bacterial Wilt Suppression in Soil Amended with Lysine
- Quantification of Pratylenchus penetrans in radish fields using a combination method of soil compaction and real-time PCR to determine the economic threshold(Soil Biology)
- Inhibition of Bacterial Wilt of Tomato Caused by Ralstonia solanacearum by Sugars and Amino Acids
- Combined Analyses of Bacterial, Fungal and Nematode Communities in Andosolic Agricultural Soils in Japan
- Specific Bacterial Community Stimulated by Lysine and Its Role in the Disease suppression of Tomato Bacterial Wilt(The annal Meeting of the Society in 2009)
- Difference of soil biological responses to amendment with okara and coffee residue compost or cow manure compost
- Mechanism of tomato bacterial wilt suppression by the amendment of lysine in soil(The annal Meeting of the Society in 2008)
- Effect of lysine combine with biocontrol agent on suppressiveness to soil-borne pathogen(The annal Meeting of the Society in 2008)
- Associated bacteria with several aquatic organisms in the floodwater of rice fields determined by PCR-RFLP(The Annual Meeting of the Society in 2000)
- Difference of soil biological responses to amendment with okara and coffee residue compost or cow manure compost
- Susceptibility of soils with or without repeated application of farmyard manure to bacterial wilt of tomato caused by Ralstonia solanacearum
- Recent Trends in Microbial Inoculants in Agriculture
- Mechanism of Tomato Bacterial Wilt Suppression in Soil Amended with Lysine