Cloning, Expression, and Identification of Genes Involved in the Conversion of DL-2-Amino-Δ^2-thiazoline-4-carboxylic Acid to L-Cysteine via S-Carbamyl-L-cysteine Pathway in Pseudomonas sp. TS1138
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概要
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Two novel genes (tsB, tsC) involved in the conversion of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC) to L-cysteine through S-carbamyl-L-cysteine (L-SCC) pathway were cloned from the genomic DNA library of Pseudomonas sp. TS1138. The recombinant proteins of these two genes were expressed in Escherichia coli BL21, and their enzymatic activity assays were performed in vitro. It was found that the tsB gene encoded an L-ATC hydrolase, which catalyzed the conversion of L-ATC to L-SCC, while the tsC gene encoded an L-SCC amidohydrolase, which showed the catalytic ability to convert L-SCC to L-cysteine. These results suggest that tsB and tsC play important roles in the L-SCC pathway and L-cysteine biosynthesis in Pseudomonas sp. TS1138, and that they have potential applications in the industrial production of L-cysteine.
- 2007-09-23
著者
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Liu Zhong
Department Of Microbiology College Of Life Sciences Nankai University
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Kuwano Eiichi
Laboratory of Pesticide Chemistry,Division of Plant Protection,Department of Applied Genetics and Pe
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SHIOTSUKI Takahiro
Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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Bai Gang
Department Of Microbiology College Of Life Sciences Nankai University
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Kuwano Eiichi
Laboratory Of Pesticide Chemistry Department Of Applied Genetics And Pest Management Faculty Of Agri
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Kuwano Eiichi
Laboratory Of Pesticide Chemistry Department Of Agricultural Chemistry Kyushu University
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FUJITA Norihiro
Laboratory of Pesticide Chemistry, Department of Applied Genetics and Pest Management, Faculty of Ag
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YAMADA Naotaka
Laboratory of Pesticide Chemistry, Department of Applied Genetics and Pest Management, Faculty of Ag
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Yamada Naotaka
Laboratory Of Pesticide Chemistry Department Of Applied Genetics And Pest Management Faculty Of Agri
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Yamada Naotaka
Laboratory Of Pesticide Chemistry Department Of Agricultural Chemistry Kyushu University
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Fujita Norihiro
Laboratory Of Pesticide Chemistry Department Of Applied Genetics And Pest Management Faculty Of Agri
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ASHIBE Kiyo
Laboratory of Pesticide Chemistry, Department of Applied Genetics and Pest Management, Faculty of Ag
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Kiuchi Makoto
Invertebrate Gene Function Research Unit National Institute Of Agrobiological Science
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Ashibe Kiyo
Laboratory Of Pesticide Chemistry Department Of Applied Genetics And Pest Management Faculty Of Agri
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Shiotsuki Takahiro
Invertebrate Gene Function Research Unit National Institute Of Agrobiological Science
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Yu Yangsheng
Laboratory of Pesticide Chemistry, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
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Liu Zhong
Laboratory of Pesticide Chemistry, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
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Liu Chunqin
Laboratory of Pesticide Chemistry, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
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