Characterization of Juvenile Hormone Epoxide Hydrolase and Related Genes in the Larval Development of the Silkworm Bombyx mori
スポンサーリンク
概要
- 論文の詳細を見る
Juvenile hormone epoxide hydrolases (JHEHs) are a family of enzymes that hydrolyze juvenile hormones (JHs). They are important in terms of organ-specific regulation and irreversible degradation. In contrast to three JHEH genes (jheh) in Drosophila melanogaster and five jheh in Tribolium castaneum, only one jheh gene has been reported to date in lepidopteran insects. By searching a genome database of the silkworm, KAIKOBLAST, five JHEH-related genes (jheh-r), in addition to Bmjheh, were found. Developmental changes in mRNA expression were brought about revealing several unique patterns for each of jheh-r as to developmental stages and organ-specificity. Recombinant proteins of JHEH-r were expressed using a baculovirus system to evaluate their enzymatic activities. Three of the five JHEH-r recombinant proteins had JH hydrolytic activities. This is the first report on lepidopteran jheh-related genes and also provides the comprehensive analysis of multiple jheh-related genes in an insect species with respect to their functions in enzyme activities.
- 社団法人 日本農芸化学会の論文
- 2010-07-23
著者
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Seino Atsushi
Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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Tsubota Takuya
Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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SHIMOMURA Masaru
Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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NAKAKURA Takayo
Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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TAN Anjiang
Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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MITA Kazuei
Insect Genome Research Unit, National Institute of Agrobiological Science
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SHINODA Tetsuro
Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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NAKAGAWA Yoshiaki
Department of Applied Life Sciences, Kyoto University
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SHIOTSUKI Takahiro
Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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Tsubota Takuya
Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science
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Seino Atsushi
Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science
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Mita Kazuei
Insect Genome Research Unit National Institute Of Agrobiological Science
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Nakagawa Yoshiaki
Department Of Applied Life Sciences Kyoto University
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Shimomura Masaru
Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science
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Nakakura Takayo
Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science
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OGURA Takehiko
Invertebrate Gene Function Research Unit, National Institute of Acrobiological Science
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Ogura Takehiko
Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science
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Tan Anjiang
Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science
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Shinoda Tetsuro
Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science
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Shiotsuki Takahiro
Invertebrate Gene Function Research Unit National Institute Of Agrobiological Science
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Mita Kazuei
Insect Genome Res. Unit National Inst. Of Agrobiological Sci.
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NAKAGAWA Yoshiaki
Department of Agricultural Chemistry, Kyoto University
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