Characterization and Analysis of Novel Carboxyl/Cholinesterase Genes Possessing the Thr-316 Motif in the Silkworm, Bombyx mori

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概要

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• Juvenile hormone esterases (JHEs) function in juvenile hormone (JH) degradation. In the silkworm, Bombyx mori, we have characterized authentic JHE (Bmjhe) and five other carboxyl/cholinesterase (CCE) genes (Bmcce-1 to -5) with GQSAG, a motif sequence of JHE. But none of the genes appeared to function in vivo as a JHE, except for Bmjhe. Recently it was reported that the GQSAG motif might be dispensable, and that the Thr-316 residue has functional significance for JHE activity. On the basis of these findings, we identified two novel JHE candidates, Bmcce-6 and Bmcce-7, that lack GQSAG but possess Thr-316. In the CCE phylogenetic tree, BmCCE-6 was close to the lepidopteran JHE cluster, while BmCCE-7 constituted the same cluster as pheromone-degrading esterases. The developmental expression profiles were different among Bmjhe, Bmcce-6, and Bmcce-7. None of the proteins hydrolyzed JH in vitro. Our results suggest that only one CCE (BmJHE) functions as JHE in the silkworm.

• 社団法人 日本農芸化学会の論文
• 2010-11-23

著者

• SHIOTSUKI Takahiro

  Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science

• Tsubota Takuya

  Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science

• Nakakura Takayo

  Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science

• Shinoda Tetsuro

  Invertebrate Gene Function Research Unit National Institute Of Acrobiological Science

• Shiotsuki Takahiro

  Invertebrate Gene Function Research Unit National Institute Of Agrobiological Science

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• Characterization of Juvenile Hormone Epoxide Hydrolase and Related Genes in the Larval Development of the Silkworm Bombyx mori
• Characterization and Analysis of Novel Carboxyl/Cholinesterase Genes Possessing the Thr-316 Motif in the Silkworm, Bombyx mori
• Molecular characterization and mRNA expression analysis of farnesyl transferase and geranylgeranyl transferase genes in the silkworm Bombyx mori
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