Pathogenic Cellulase Assay of Pine Wilt Disease and Immunological Localization

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概要

• 論文の詳細を見る

• The pine wilt disease caused by Bursaphelenchus xylophilus (BX), also known as the pine wood nematode (PWN), is the most devastating disease of pine trees. In this work, a high molecular weight B. xylophilus cellulase antigen (BXCa) was purified from total homogenates of nematodes. BXCa was found to be able to hydrolyze carboxymethyl cellulose (CMC) efficiently (155.65 U/mg) and to have an approximate molecular mass of 58.9 kDa. We harvested anti-BXCa antibodies and performed immunocytochemical assays, which revealed the localization of cellulase pools in the esophageal gland cells of the PWN. It was also discovered that cellulase was secreted from the stylet and was used to hydrolyze cellulose to facilitate the PWN entering host cells. These results are consistent with other plant parasitical nematodes. Interestingly, strong fluorescence signals from cellulase staining were observed in tracheid cells in naturally infected pine wood, in addition to ray cells and the resin canal zone. These results strongly suggest that the cellulase released by the PWN is one of the pathogenic substances of pine wilt disease and is responsible for the development of the early symptoms of the disease.

• 社団法人 日本農芸化学会の論文
• 2006-11-23

著者

• ZHANG Qi

  Department of Cardiology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine

• Bai Gang

  Department Of Microbiology College Of Life Sciences Nankai University

• Zhang Qi

  Department Of Cardiology Rui Jin Hospital Shanghai Jiao Tong University School Of Medicine

• Li Haiyan

  General Station Of Forest Pest And Disease Control The State Administration Of Forestry

• YANG Wenbo

  Department of Microbiology, College of Life Sciences, Nankai University

• XIONG Huilong

  General Station of Forest Pest and Disease Control, The State Administration of Forestry

• Zhang Qi

  Department Of Microbiology College Of Life Sciences Nankai University

• Yang Wenbo

  Department Of Microbiology College Of Life Sciences Nankai University

• Xiong Huilong

  General Station Of Forest Pest And Disease Control The State Administration Of Forestry

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