Radiation-induced Enhancement of Enzymatic Cell Lysis of Micrococcus radiodurans
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概要
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The intact cells of M. radiodurans were rendered sensitive to the action of lytic enzyme (P2-2 enzyme) by irradiation. The radiation-induced enhancement of cell lysis with P2-2 enzyme was completely prevented by the addition of t-butanol and irradiation at liquid nitrogen temperature. These results indicate that the enhancement is due to indirect action resulting from OH radicals. Cell lysis by lysozyme was enhanced only when the cells were irradiated under N2O. The enhancement of cell lysis with lysozyme was also prevented by adding alcohols. On the other hand, when lipid components in cells were removed by extraction with n-butanol, the radiation-induced enhancement of cell lysis with P2-2 enzyme and lysozyme was not observed. From these results it is concluded that the enhancement of enzymatic cell lysis by irradiation is attributable to alteration in the lipid-rich layer of the cell wall caused by OH radicals.
- 社団法人 日本農芸化学会の論文
著者
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WATANABE Hiroshi
Takasaki Radiation Chemistry Establishment, Japan Atomic Enerty Research Institute (JAERI)
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Takehisa Masaaki
Takasaki Radiation Chemistry Research Establishment Japan Atomic Energy Research Institute
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IIZUKA Hiroshi
Institute of Applied Biology, Science University of Tokyo
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