Effect of Gamma-Irradiation on Purified Glucose Isomerase in Dilute Solution
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概要
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Glucose isomerase purified from cells of Streptomyces phaeochromogenus was irradiated in dilute solution, and the effect of γ-irradiation was investigated. The activity of the enzyme decreased exponentially with the dose under all conditions investigated. The inactivation yields (G0-value) in neutral solution were 0.069 in air and 0.115 in nitrogen. The role of the radicals produced by water radiolysis in the inactivation of glucose isomerase was estimated by using nitrous oxide tert-butanol as selective radical scavengers. Under these conditions, the hydroxyl radical and the hydrogen atom were found to be important in the enzyme inactivation, and the hydrated electron contributed very little. The inactivation efficiencies of the hydroxyl radical, the hydrogen atom and the hydrated electron were 0.032, 0.025 and 0.005, respectively.
- 社団法人 日本農芸化学会の論文
著者
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WATANABE Hiroshi
Takasaki Radiation Chemistry Establishment, Japan Atomic Enerty Research Institute (JAERI)
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Kume Tamikazu
Takasaki Radiation Chemistry Research Establishment Japan Atomic Energy Research Institute
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AOKI Shohei
Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute
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SATO Tomotaro
Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute
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SATO Tomotaro
Takasaki Radiation Chemistry Research Establishment, Japan A tomic Energy Research Institute
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