Isolation and Fundamental Properties of enofo-Pectate Lyase pl-Isozymes from Erwinia carotovora
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概要
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A strain of Erwinia carotovora was found to produce extracellularly four kinds of endo-pectate lyase pl-isozyme (PATE-I, -II, -III and -IV) having similar properties. The four pl-isozymes were purified to homogenous states by ion exchange chromatography, gel nitration and isoelectric focusing. The approximate molecular weights were 28, 000 for PATE-I and -III, 32, 000 for PATE-II and 33, 000 for PATE-IV, respectively, and they had isoelectric points of 10-11. The optimum pHs for the reaction catalyzed by these pl-isozymes were 9.3 for PATE-II, 9.5 for PATE-IV and 9.7 for PATE-I and III, respectively. There were no differences in the optimum Ca2+ concentration (0.5-0.6mM) or Km and Vmax among these four pl-isozymes. The mode of action of all the plisozymes was determined to be the endo-type.
- 社団法人 日本農芸化学会の論文
著者
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Wada Fumio
Department Of Endocrinology Faculty Of Medicine Kagawa Medical University
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Kobayashi Yoshinari
Government Industrial Research Institute
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TANABE Hiroyuki
Government Industrial Research Institute
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Nishi Nozomu
Department Of Endocrinology Faculty Of Medicine Kagawa Medical University
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Matuo Yuhsi
Department Of Internal Medicine And Molecular Science Graduate School Of Medicine Osaka University
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MATUO Yuhsi
Department of Endocrinology, Kagawa Medical School
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