Production of Pectolytic Enzymes from Erwinia carotovora and Their Stability : Biochemical Pulping (XIV)

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For the application of pectolytic enzymes, mainly endo-pectate lyase (endo-PATE), secreted by Erwinia carotovora to biochemical pulping of bast fibers, factors governing the production of the enzymes were investigated to obtain the optimum cultivation conditions. The bacterium required pectin, especially citrus pectin, as an essential inducible carbon source and asparagine as a nitrogen source for improvement of endo-PATE productivity. The requirement for phosphate was comparatively low, ranging from 3.3 to 5.0 mM. A slightly acidic pH range (pH 6.0±0.2) of the medium, strong aeration, and appreciably lower temperature (22℃) gave higher productivity of the endo-PATE and endo-polygalacturonase. There was a time discrepancy between the maximum growth and the optimum production of the enzymes. The endo-PATE has the optimum temperature of 50℃ at the optimum pH 9.5 but was almost deactivated within 1 h under these conditions. Lowering the incubation temperature to 30℃ or less and the addition of Ca^<2+> up to 5 mM improved the stability.
1985-10-25