ツツジ科植物からのdark septate endophyteの分離とそれらの分子系統関係

概要

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A total of 91 isolates of dark septate endophytes( DSE) were isolated from the family Ericaceae plants. InternalTranscribed Spacer (ITS) and 5.8S rDNA sequence were determined in 10 non-pathogenic isolates of DSE,including a Heteroconium chaetospira. The determined sequences were subjected to Neighbor-joining analysis ofMEGA4. We identified Heteroconium chaetospira( syn. Cladophialophora chaetospira) on the first time in Japanfrom Ericaceae plant. Three isolates had a close relationship with Leptodontidium orchidicola. Identities of nonDSE isolates like Pj029 and Ro034 were still unknown and remain so until isolates sporulate or until more taxawill be sequenced. Strong bootstrap support within the phylogenetic tree between the Pleosporales isolates andPj022 confirmed their congeneric status, while Ro24 and Ro012 were exhibited close affinity to Cyphellophora sp.All DSE isolates had the ability to form intercellular structures within the epidermal cells of blueberry( Vacciniumcorymbosum L.).
2011-03-00