発生に伴うオカダンゴムシ造雄腺ホルモン（AGH）の発現動態の解析
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概要
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Male sexual characteristics in crustaceans are induced by androgenic gland hormone (AGH), which is produced in a male-specific organ, the androgenic gland (AG). In this study, protein and mRNA expression of AGH in AGs of the juvenile terrestrial isopod Armadillidium vulgare were examined using immunohistochemistry and RT-PCR, respectively. AGs of 7th and 8th instar larvae were immunostained with an anti-A. vulgare AGH antibody, although no positive reaction was observed in the AGs of 5th and 6th larval stages. On the other hand, AGH mRNA was detected in all subjected larval stages (2nd to 8th instar larvae). Amplified cDNA was detected as a faint band in 2nd to 4th instar larvae and as a thick band in 5th to 8th larval stages. This discrepancy of protein and mRNA expression may be due to the detection sensitivities for AGH of immunohistochemistry and RT-PCR. These results suggest that AGH regulates the development of male characteristics at least from the 7th larval stage.
- 2010-06-30
著者
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長澤 寛道
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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