Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis
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概要
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Echinococcus multilocularis causes an important zoonotic cestode disease. The metacestocle stage proliferates in the liver of intermediate hosts including human and rodents and forms multiple cysts. Recently, members of a transmembrane protein tetraspanin (TSP) family have been used as vaccines against schistosomosis, or as diagnostic antigens for cysticercosis. In this study, seven tetraspanins of E. multilocularis, designated as TSP1 to TSP7, were evaluated for their protective potential against primary alveolar echinococcosis. The large extracellular loop (LEL) region of these tetraspanins was cloned from a full-length enriched cDNA library of E. multilocularis metacestodes and expressed in Escherichia coli as a fusion protein with thioredoxin. Recombinant TSPs were applied as vaccines against an E. multilocularis primary experimental infection in BALB/c mice. Cyst lesions in the livers of vaccinated and non-vaccinated mice were counted. The cyst lesion reduction rates induced by the seven tetraspanins in vaccinated vis-à-vis non-vaccinated mice were: 87.9%, 65.8%, 85.1%, 66.9%, 73.7%, 72.9% and 37.6%. Vaccination conferred protective rates to mice ranging from 0% (TSP5, 6, 7) to maximally 33% (TSP1, 3). The results indicated that recombinant tetraspanins have varying protective effects against primary alveolar echinococcosis and could be used in vaccine development.
- 2009-12-09
著者
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八木 欣平
北海道衛研
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八木 欣平
北海道立衛生研究所
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八木 欣平
北海道大学獣医学部家畜寄生虫病学講座
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Yagi Kinpei
Hokkaido Institute Of Health
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Yagi Kinpei
Department Of Parasitology Faculty Of Veterinary Medicine Hokkaido University
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Yagi Kinpei
Department Of Biology Hokkaido Institute Of Public Health
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Matsumoto Jun
Laboratory Of Parasitology Department Of Disease Control Graduate School Of Veterinary Medicine Hokk
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Oku Yuzaburo
Laboratory Of Parasitology Department Of Disease Control Graduate School Of Veterinary Medicine Hokk
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Kouguchi Hirokazu
Department Of Biological Science Hokkaido Institute Of Public Health
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