Two-component bacterial multidrug transporter, EbrAB: Mutations making each component solely functional
スポンサーリンク
概要
- 論文の詳細を見る
EbrAB in Bacillus subtilis belongs to a novel small multidrug resistance (SMR) family of multidrug efflux pumps. EmrE in Escherichia coli, a representative of SMR, functions as a homo-oligomer in the membrane. On the other hand, EbrAB requires a hetero-oligomeric configuration consisting of two polypeptides, EbrA and EbrB. Although both polypeptides have a high sequence similarity, expression of either single polypeptide does not confer the multidrug-resistance. We performed mutation studies on EbrA and B to determine why EbrAB requires the hetero-oligomerization. Mutants of EbrA and B lacking both the hydrophilic loops and the C-terminus regions conferred the multidrug-resistance solely by each protein. This suggests that the hydrophilic loops and the C-terminus regions constrain them to their respective conformations upon the formation of the functional hetero-oligomer.
- Elsevier B.V.の論文
著者
-
Kouno T
Faculty Of Pharmaceutical Sciences University Of Toyama
-
水谷 忠士
Faculty Of Advanced Life Science Hokkaido University
-
Kikukawa T
Recording Media And Solutions Business Group Tdk Corporation
-
Miyauchi Seiji
Faculty Of Pharmaceutical Science Matsuyama Univ.
-
水谷 忠士
Fac. Of Advanced Life Sci. Hokkaido Univ.
-
Miyauchi Seiji
Matsuyama University
-
Nara Toshifumi
College Of Pharmaceutical Sciences Matsuyama Univ
-
Kamo Naoki
Faculty Of Advanced Life Science Hokkaido University
-
Kamo Naoki
Laboratory Of Biophysical Chemistry Faculty Of Advanced Life Science Hokkaido University
-
Kamo Naoki
Faculty Of Pharmaceutical Sciences Grad. School Of Pharmaceutical Siences Hokkaido Univ.
-
Kamo Naoki
College Of Pharmaceutical Science Matsuyama University Matsuyama Japan
関連論文
- Heterologous Expression of Pharaonis Halorhodopsin in Xenopus laevis Oocytes and Electrophysiological Characterization of Its Light-Driven Cl- Pump Activity
- Interaction of the Halobacterial Transducer to a Halorhodopsin Mutant Engineered so as to Bind the Transducer : Cl- Circulation within the Extracellular Channel
- Participation of the surface structure of pharaonis phoborhodopsin, ppR and its A149S and A149V mutants, consisting of the C-terminal α-helix and E-F loop, in the complex-formation with the cognate transducer pHtrII, as revealed by site-directed 13C solid
- Importance of specific hydrogen bonds of archaeal rhodopsins for the binding to the transducer protein†
- 2P313 Structural network in the extracellular region corresponding to the visual spectral shift of halorhodopsin(41. Proton and ion pumping,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P311 The role of E234 on the extracellular site in the anion pump of pharaonis halorhodopsin(41. Proton and ion pumping,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Role of Arg-72 of pharaonis Phoborhodopsin (sensory rhodopsin II) on its photochemistry.
- Role of Asp193 in chromophore-protein interaction of pharaonis phoborhodopsin (sensory rhodopsin II).
- Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.
- Tyr-199 and charged residues of pharaonis Phoborhodopsin are important for the interaction with its transducer.
- Association of pharaonis phoborhodopsin with its cognate transducer decreases the photo-dependent reactivity by water-soluble reagents of azide and hydroxylamine.
- Selective reaction of hydroxylamine with chromophore during the photocycle of pharaonis phoborhodopsin.
- 1P423 Local Conformation and Dynamics Changes in the vicinity of the Retinal in Photoactivated pharaonis phoborhodopsin by Solid-State NMR(17. Light driven system,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- 3P-097 ハロロドプシンの光活性はホモ三量体化によって変調を受けるのか?(膜蛋白質,第46回日本生物物理学会年会)
- 2P-264 大腸菌発現系を用いたsalinarumハロロドプシンの機能解析(光生物・視覚,光受容(2),第46回日本生物物理学会年会)
- 2P-259 光駆動型クロライドポンプハロロドプシンのアニオン取込みにおけるアルギニン側鎖の構造変化(光生物・視覚,光受容(2),第46回日本生物物理学会年会)
- The Solution Structure of Diapause-Specific Peptide
- Dimeric Solution Structure of Antimicrobial Peptide Tachystatin B with an Inhibitory Cystine-Knot Motif
- 3P-217 大腸菌多剤排出輸送担体EmrEのアンチパラレル構造(生体膜/人工膜・輸送,第46回日本生物物理学会年会)
- 3P-216 ハロロドプシンポンプ機能におけるシッフ塩基近傍酸性残基の役割(生体膜/人工膜・輸送,第46回日本生物物理学会年会)
- Anti-parallel membrane topology of two components of EbrAB, a multidrug transporter.
- 1P512 Fast backbone amide assignment of ^N-HSQC spectrum(25. New methods and tools (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Two-component bacterial multidrug transporter, EbrAB: Mutations making each component solely functional
- A light-driven proton pump from Haloterrigena turkmenica: Functional expression in Escherichia coli membrane and coupling with a H+ co-transporter
- Culture Temperature Affects the Molecular Motion of Bacteriorhodopsin within the Purple Membrane
- Observation of Eye Pattern on Super-Resolution Near-Field Structure Disk with Write-Strategy Technique
- Super-Resolution Read-Only Memory Disk with Metal Nanoparticles or Small Aperture
- Super-Resolution Near-Field Structure and Signal Enhancement by Surface Plasmons
- 2P-274 ^C固体NMRによるppRと複合体を形成したpHtrIIの光活性化状態における運動と構造変化の解析(光生物・視覚,光受容(2),第46回日本生物物理学会年会)
- 2P-260 ^C固体NMRによるppRの細胞質表面部位の相互作用変化の観測(光生物・視覚,光受容(2),第46回日本生物物理学会年会)
- 1P427 Local structural change of bacteriorhodopsin accompanied with the removal of retinal as studied by solid state NMR(17. Light driven system,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- 1P426 Local structure analysis of Tyr residues is located in transmembrane site of bacteriorhodopsin by solid state high resolution NMR(17. Light driven system,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- 1P425 Structure analysis of Tyr residues with retinal isomerization in bacteriorhodopsin by solid state NMR(17. Light driven system,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells
- Studies on Effects of External Electric Fields on Halobacteria with Fluorescence Intensity and Fluorescence Lifetime Imaging Microscopy
- A Correlation between pH and Fluorescence Lifetime of 2',7'-Bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) In Vivo and In Vitro
- 3P002 Conformational changes of extracellular loop of halorhodopsin by chloride binding(Protein: Structure,The 48th Annual Meeting of the Biophysical Society of Japan)
- 2P272 海藻由来アセタブラリアロドプシンIIの光化学反応(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 1P255 ハロロドプシンのCl-移動中における,シッフ塩基のpKa変化(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 1P119 多剤輸送担体EmrEと基質とのpH依存的相互作用様式の変化(膜蛋白質,第48回日本生物物理学会年会)
- 1P262 負の走光性レセプターNpSRIIと大腸菌走化性レセプターTsrの機能的相互作用(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 2P088 無脊椎動物由来抗菌ペプチドと脂質膜の相互作用解析(蛋白質-機能(反応機構,生物活性など),第48回日本生物物理学会年会)
- 1P083 ヒト由来機能未知タンパク質ZNF821における23アミノ酸からなるタンデムリピートドメインSTPRの機能解析(蛋白質-機能(反応機構,生物活性など),第48回日本生物物理学会年会)
- 3P274 A Long-lived photoproduct, P370, formed from salinarum phoborhodopsin at room temperature(Photobiology: Vision & Photoreception,The 48th Annual Meeting of the Biophysical Society of Japan)
- 3P-209 ファラオニスハロロドプシンのCl-輸送機構におけるAsp252の役割(光生物-視覚・光受容,第47回日本生物物理学会年会)
- 3P-208 Halobacterium salinarum由来のフォボロドプシン(センサリーロドプシンII)の光化学反応サイクルとプロトン移動(光生物-視覚・光受容,第47回日本生物物理学会年会)
- 3P-205 大腸菌発現系を用いたsalinarumハロロドプシンの機能的再構成(光生物-視覚・光受容,第47回日本生物物理学会年会)
- The Structure of Physarum polycephalum Hemagglutinin I Suggests a Minimal Carbohydrate Recognition Domain of Legume Lectin Fold
- 1P-261 高度好塩菌フォボロドプシン(センサリーロドプシンII)の光反応サイクル(光生物・視覚,光受容(1),第46回日本生物物理学会年会)
- COMPARATIVE ANALYSIS OF HEPATIC UPTAKE AND BILIARY EXCRETION OF VINCRISTINE AND VINBLASTINE IN PERFUSED RAT LIVER
- KINETICS OF HEPATIC TRANSPORT OF 4-METHYLUMBELLIFERONE IN RATS : ANALYSIS BY MULTIPLE INDICATOR DILUTION METHOD
- 3P266 光照射固体NMRによるファラオニスフォボロドプシンの信号伝達に関わるTyr174の局所構造変化の解析(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 3P265 光照射固体NMRを用いたppRのM中間体の観測と光異性化によるレチナール化学シフト変化の解析(光生物-視覚・光受容,第48回日本生物物理学会年会)
- Roles of Ser130 and Thr126 in Chloride Binding and Photocycle of pharaonis Halorhodopsin
- 2P-083 SMR型多剤排出輸送担体と基質の相互作用に伴う熱量変化(膜蛋白質,第47回日本生物物理学会年会)
- 1P-032 新規デフェンシンの膜構造と抗菌活性(蛋白質-構造機能相関,第47回日本生物物理学会年会)
- 1TA1-09 新規デフェンシンの膜構造と抗菌活性(蛋白質-構造機能相関,第47回日本生物物理学会年会)
- 3P-207 ファラオニスフォボロドプシンの光反応サイクルに対するpHの影響(光生物-視覚・光受容,第47回日本生物物理学会年会)
- 1P118 Structural stability and photocycle of bacteriorhodopsin in short chain phospholipid micelles(Membrane proteins,The 48th Annual Meeting of the Biophysical Society of Japan)
- 3P279 膜タンパク質ハロロドプシンの構造安定性と機能へのヘリックス間ループの影響(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 3P100 共発現による封入体化を利用した効率的な短鎖ペプチド生産技術(蛋白質-蛋白質工学/進化工学,第48回日本生物物理学会年会)
- 3P006 タキプレシンI-LPS複合体の立体構造解析(蛋白質-構造,第48回日本生物物理学会年会)
- 1P264 ハロロドプシンの三量体形成メカニズムと機能変調(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 1P027 細胞増殖抑制活性を持つ新規誘導体の構造および相互作用の解析(蛋白質-構造,第48回日本生物物理学会年会)
- 3P-096 蛍光相関分光法を用いたFMBP-1の生体内分子動態解析(核酸結合蛋白質,第47回日本生物物理学会年会)
- 2P-084 ハロロドプシンNpHRの三量体構造はF150残基の対称配置によって安定化される(膜蛋白質,第47回日本生物物理学会年会)
- 1P-076 共発現によるペプチド封入体化発現法におけるパートナータンパク質の検討(蛋白質-蛋白質工学/進化工学,第47回日本生物物理学会年会)
- 2TP2-01 ハロロドプシンNpHRの三量体構造はF150残基の対称配置によって安定化される(膜蛋白質,第47回日本生物物理学会年会)
- 1TP5-09 機能未知ヒト蛋白質の23アミノ酸繰返しドメインのDNA結合性について(核酸結合蛋白質,第47回日本生物物理学会年会)
- 魚醤の化学分析と「うま味」の文化圏 : 魚の発酵製品の研究(6)
- Anti-parallel Membrane Topology of a Homo-dimeric Multidrug Transporter, EmrE
- The Extracellular pH Dependency of Transport Activity by Human Oligopeptide Transporter 1 (hPEPT1) Expressed Stably in Chinese Hamster Ovary (CHO) Cells : A Reason for the Bell-Shaped Activity versus pH(Biopharmacy)
- The elution profile of immobilized liposome chromatography: determination of association and dissociation rate constants.
- 1P-250 全反射赤外分光法によるファラオニスフォボロドプシンのプロトン放出機構の研究(光生物・視覚,光受容(1),第46回日本生物物理学会年会)
- 1P-218 固体^C NMRによるppRの細胞質表面部位の相互作用変化の観測(光生物-視覚・光受容,第47回日本生物物理学会年会)
- 1P-217 固体NMRによる光受容タンパク質ppRとその変異体T204Aの局所構造変化の解析(光生物-視覚・光受容,第47回日本生物物理学会年会)
- 1TP2-01 固体NMRによる光受容タンパク質ppRとその変異体T204Aの局所構造変化の解析(光生物-視覚・光受容,第47回日本生物物理学会年会)
- 1P420 Studies on reaction dynamics of halorhodopsin during chloride ion pumping(17. Light driven system,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- Contribution of thermal desorption and liquid–liquid extraction for identification and profiling of impurities in methamphetamine by gas chromatography–mass spectrometry
- Effect of Molecular Assembly on Photocycle of Reconstituted Bacteriorhodopsin : Significant Blue Shift of the Late M Photointermediate in the Liquid Crystalline Phase
- Analysis of amphetamine-type stimulants and their metabolites in plasma, urine and bile by liquid chromatography with a strong cation-exchange column-tandem mass spectrometry
- Conformational analysis of novel peptide derivatives with cytostatic activity
- Expression of salinarum halorhodopsin in Escherichia coli cells : Solubilization in the presence of retinal yields the natural state
- 2G1448 線虫由来抗菌ペプチドABF-2の発現、精製と立体構造解析(蛋白質_構造2,第49回日本生物物理学会年会)
- 2G1436 植物由来抗菌ペプチドsnakin-1の立体構造解析へ向けた研究(蛋白質_構造2,第49回日本生物物理学会年会)
- 2D1612 抗菌ペプチドhuman defensin 5前駆体の大量発現とその解析(蛋白質_構造機能相関1,第49回日本生物物理学会年会)
- 2Q1636 低pH条件下におけるプロテオロドプシンの速いプロトン放出について(光生物_視覚・光受容2,第49回日本生物物理学会年会)
- 1B1512 Decay kinetics of the M photointermediate of bacteriorhodopsin in short chain phosphatidylcholine micelles(Membrane Proteins 1,The 49th Annual Meeting of the Biophysical Society of Japan)
- 3Q0948 光照射固体NMRを用いたppR/oHtrII複合体の光活性化に伴う構造変化の解析(光生物_視覚・光受容3,第49回年会講演予稿集)
- 3Q0936 Photo-induced dynamics change of phoborhodopsin with transducer protein as studied by in-situ photo irradiated solid-state NMR(Photobiology : Vision & Photoreception3,The 49th Annual Meeting of the Biophysical Society of Japan)
- 1B1536 ハロロドプシンの三量体安定化に対するSer^の役割(膜蛋白質1,第49回日本生物物理学会年会)
- 1B1524 P36 atronomonas pharaonis HalorhodopsinのCl-輸送に伴う体積変化(膜蛋白質1,第49回日本生物物理学会年会)
- 1B1448 部分フッ素化フォスファチジルコリン小胞へのバクテリオロドプシンの再構成(膜蛋白質1,第49回日本生物物理学会年会)
- Structural Analysis of Plant Antimicrobial Peptide Snakin-1
- NMR Structure and Interaction of Antimicrobial Peptide Tachyplesin I with Lipopolysaccharide
- Construction of a Novel Expression System for Cryptdin-4 by Using Inclusion Body Formation
- Does Escherichia coli Thioredoxin Improve Expression Efficiency of Recombinant Peptide in Pichia pastoris?
- Expression, Purification and NMR Characterization of the Cyclic Reconbinant Form the First Extracellular Loop of the Transmembrane Protein Halorhodopsin
- Expression and Structural Analysis of a Recombinant Antibacterial Peptide, Cecropin P1
- 2PT176 Natronomonas pharaonis由来のセンサリーロドプシンIIの光誘起プロトン移動における塩化物イオンの役割(日本生物物理学会第50回年会(2012年度))