Expression of salinarum halorhodopsin in Escherichia coli cells : Solubilization in the presence of retinal yields the natural state

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概要

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• Salinarum halorhodopsin (HsHR), a light-driven chloride ion pump of haloarchaeon Halobacterium salinarum, was heterologously expressed in Escherichia coil. The expressed HsHR had no color in the E. coli membrane, but turned purple after solubilization in the presence of all-trans retinal. This colored HsHR was purified by Ni-chelate chromatography in a yield of 3-4 mg per liter culture. The purified HsHR showed a distinct chloride pumping activity by incorporation into the liposomes, and showed even in the detergent-solubilized state, its typical behaviors in both the unphotolyzed and photolyzed states. Upon solubilization, HsHR expressed in the E. coli membrane attains the proper folding and a trimeric assembly comparable to those in the native membranes.

著者

• Kuwajima Kunihiro

  Okazaki Institute For Integrative Bioscience National Insitutes Of Natural Science:dept Of Functiona

• Kuwajima Kunihiro

  東京大学 物理学専攻

• Demura M

  Graduate School Of Life Science Hokkaido University

• Demura Makoto

  東京大学 物理学専攻

• Demura Makoto

  Division Of Molecular Life Sciences Faculty Of Advanced Life Science. Hokkaido University

• Demura Makoto

  Graduate School Of Science Hokkaido University

• Demura Makoto

  Division Of Biological Sciences Graduate School Of Science Hokkaido University

• Kawano K

  Graduate School Of Life Science Hokkaido University

• Kawano Keiichi

  東京大学 物理学専攻

• Aizawa Tomoyasu

  Graduate School Of Life Science Hokkaido University

• Aizawa Tomoyasu

  東京大学 物理学専攻

• Demura Makoto

  Div. Of Biological Science Grad. School Of Science Hokkaido Univ.

• KAMIYA Masakatsu

  Graduate School of Life Science, Hokkaido University

• Kouno T

  Faculty Of Pharmaceutical Sciences University Of Toyama

• 水谷 忠士

  Faculty Of Advanced Life Science Hokkaido University

• Miyauchi Seiji

  Faculty Of Pharmaceutical Science Matsuyama Univ.

• Kamiya Masakatsu

  Graduate School Of Life Science Hokkaido University

• Kamiya Masakatsu

  Division Of Biological Sciences Graduate School Of Science Hokkaido University

• Miyauchi Seiji

  Matsuyama University

• Kohno Takahide

  Faculty Of Pharmaceutical Sciences Toyama University

• Kamo N

  Hokkaido Univ. Sapporo Jpn

• Kamo Naoki

  Department Of Pharmaceutical Sciences Hokkaido University

• Kamo Naoki

  Faculty Of Advanced Life Science Hokkaido University

• Kamo Naoki

  Laboratory Of Biophysical Chemistry Faculty Of Advanced Life Science Hokkaido University

• Kawano Keiichi

  Division Of Biological Sciences Graduate School Of Science Hokkaido University

• Demura Makoto

  Hokkaido University

• Mitaku Shigeki

  Department Of Applied Physics Nagoya Univ.

• Kamo Naoki

  Faculty Of Pharmaceutical Sciences Grad. School Of Pharmaceutical Siences Hokkaido Univ.

• Kamo Naoki

  College Of Pharmaceutical Science Matsuyama University Matsuyama Japan

• Demura Makoto

  Faculty Of Advanced Life Science Hokkaido University Sapporo Japan

• Tsukamoto Takashi

  Graduate School Of Life Science Hokkaido University

• Kikukawa Takashi

  Laboratory Of Biomolecular Systems Creative Research Initiative 'sosei' (cris) Hokkaido Un

• Kuwajima Kunihiro

  Okazaki Institute For Integrative Bioscience:department Of Functional Molecular Science School Of Ph

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