Gene expression profile of bovine bone marrow mesenchymal stem cell during spontaneous chondrogenic differentiation in pellet culture system
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概要
- 論文の詳細を見る
Bovine bone marrow mesenchymal stem cells（MSCs）cultured in condensateculture, spontaneous and independent for any external biostimulants, undergochondrogenic differentiation. In the present study, the bovine MSCchondrogenesis pathway was studied by analyzing stage-specific gene expressionusing quantitative“Real Time”reverse transcriptase polymerase chainreaction（qRT-PCR）．Results showed that bovine MSCs underwent completechondrogenesis ; the initial stage was characterized by expression of sox９messenger ribonucleic acid（mRNA），followed by high transcription of chondrocytespecific genes, collagen type II and IX, biglycan and cartilage oligomericmatrix protein, and the final prehypertrophic and/or hypertrophicstage was distinguished by increased expression of collagen type X. From day７to day１４of differentiation increased mRNA expression of the transforminggrowth factors β１and β２，basic fibroblast growth factor（FGF 2），bone morphogenicprotein６（BMP 6），insulin-like growth factors１，parathyroid hormonerelated peptide and indian hedgehog（Ihh）were detected. These results suggestthat these well know chondrogenic growth factors may play a role in bovinechondrogenesis in autocrine and/or paracrine manner. On day２１of theculture, FGF 2，BMP 6 and Ihh were highly expressed, compared to cells culexterturedin monolayer manner, which suggests a possible function in maintainingthe terminal stage of differentiation. This data extends our knowledge aboutthe unusual species-specific bovine MSC chondrogenesis, allowing us to definethe phenotype of the differentiated cells. Furthermore, this study contributesto our in understanding of known chondrogenic-growth factors in autocrineand/or paracrine manner playing a role in the spontaneous differentiation．
- The Graduate School of Veterinary Medicine, Hokkaido Universityの論文
- 2006-02-28
著者
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Kim Gonhyung
Chungbuk National Univ. Chungbuk
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Kim Gonhyung
北海道大学 獣医学研究科獣医外科学
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ボスナコフスキー ダルコ
北海道大学大学院獣医学研究科診断治療学講座獣医外科学教室
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Kim Gonhyung
Chunbuk National Univ. Cheongju Kor
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BOSNAKOVSKI Darko
北大・獣医・外科
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Fujinaga T
Laboratory Of Veterinary Surgery Department Of Veterinary Clinical Sciences Graduate School Of Veter
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Fujinaga Toru
Hokkaido Univ. Sapporo Jpn
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Fujinaga Toru
Laboratory Of Veterinary Surgery Department Of Veterinary Clinical Sciences Graduate School Of Veter
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Takagi Satoshi
Laboratory Of Veterinary Surgery Department Of Veterinary Clinical Sciences Graduate School Of Veter
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Okumura Masahiro
Hokkaido Univ. Hokkaido Jpn
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Okumura M
Laboratory Of Veterinary Surgery Department Of Veterinary Clinical Sciences Graduate School Of Veter
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