The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions

概要

論文の詳細を見る
Interactions between the major proteins of Clover yellow vein virus (ClYVV) were investigatedusing a GAL4 transcription activator-based yeast two-hybrid system (YTHS). Self-interactionsmanifested by VPg and HCPro and an interaction between NIb and NIaPro were observedin ClYVV. In addition, a strong HCPro–VPg interaction was detected by both YTHS and byin vitro far-Western blot analysis in ClYVV. A potyvirus HCPro–VPg interaction has not beenreported previously. Using YTHS, domains in ClYVV for the VPg self-interaction and theHCPro–VPg interaction were mapped. The VPg C-terminal region (38 amino acids) wasimportant for the VPg–VPg interaction and the central 19 amino acids were needed for theHCPro–VPg interaction.
Society for General Microbiologyの論文