S022015 局所的な力学刺激に対する骨細胞の一酸化窒素産生挙動([S02201]細胞および分子のマイクロ・ナノスケール解析(1))

概要

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In adaptive bone remodeling, it is believed that osteocytes embedded in calcified bone matrix have an important role as a mechanosensor. Osteocytes sense mechanical stimulus and transmit biochemical signals to the cells such as osteoblasts, osteoclasts and lining cells on bone surface. In this process, nitric oxide is one of essential biochemical signals to regulate bone resorption and formation. Besides, osteocytes have characteristic stellate shape with slender processes that is different from osteoblasts. Therefore, we postulated that the osteocytic characteristic shape is important in sensing mechanical stimulus. In this study, we observed nitric oxide production induced by mechanical stimulus in osteocytes isolated from calvaria of chick embryos. Nitric oxide production is known to be caused by two different pathways: one is dependent on calcium ion, and another is independent from that. In our experiment, first, we found a characteristic oscillation of nitric oxide production in calcium-independent pathway. Second, we applied mechanical stimulus to the cell process and cell body of osteocytes independently to compare their difference in nitric oxide production. As a result, we found that cell process is more sensitive to mechanical stimulus than cell body.
一般社団法人日本機械学会の論文
2011-09-11