植物切断組織の癒合における植物ホルモンおよび転写因子の役割(奨励賞,受賞業績)

概要

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In higher plant, when wounding or grafting interrupted the original organ or tissue connection, cell division was induced and tissue-reunion occurs to restore physiological connections. Our previous work suggests that leaf gibberellin is required for the tissue-reunion of cortex in the cucumber and tomato cut hypocotyls. We identified the tissue reunion-responsive genes using Arabidopsis cut flowering stem. Genes that were up-regulated during the reunion process include those involved in cell division, cell wall modification, phytohormone-related gene and transcription factors (TFs). Among them, two plant-specific TF genes, ANAC071 and RAP2.6L, were abundantly expressed. ANAC071 was expressed at 1-3 days after cutting exclusively in the upper region of the cut gap with concomitant accumulation of indole-3-acetic acid. In contrast, RAP2.6L was expressed at 1 day after cutting exclusively in the lower region with concomitant deprivation of indole-3-acetic acid. The expression of ANAC071 and RAP2.6L were also promoted by ethylene and jasmonic acid, respectively. Our results suggested that plant-specific TFs differentially expressed around the cut position were essential for tissue reunion of Arabidopsis wounded flowering stems, and were under opposite control by polar - transported auxin, with modification by two wound-inducible hormones.
2013-05-31