Culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Paecilomyces japonica in liquid culture(BIOCHEMICAL ENGINEERING)

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概要

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• In this study, the liquid culture conditions were optimized for maximal production of mycelial biomass and exo-polysaccharide by Paecilomyces japonica. The effects of medium composition, C/N ratio and physical parameters were investigated. From these experiments, 30 g glucose, 20 g yeast extract, 0.5 g KH_2PO_4, and 0.1 g CuCl_2 2H_2O in 1-l distilled water were found to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature, initial pH, agitation, and aeration were determined to be 27℃, uncontrolled pH, 400 rpm, and 1.0 vvm, respectively. Under these optimal conditions, the maximum mycelial growth and polysaccharides production were 23.1 g/l and 2.5 g/l, respectively.

• 公益社団法人日本生物工学会の論文
• 2013-04-00

著者

• Hong Eock

  Department Of Bioengineering And Technology College Of Engineering Kangwon National University

• Park Seok

  Department Of Dermatology Seoul National University College Of Medicine

• SHIN Won

  Department of Bioengineering and Technology, Kangwon National University

• Shin Won

  Department Of Bioengineering And Technology Kangwon National University

• Lee Jong

  Depart. Of Oncology Yonsei Univ. Wonju College Of Medicine

• Jung Woo

  Department of Chemical Engineering, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea

• Lee Keun

  Department of Materials Engineering, Yonsei University, Seoul 120-749, Republic of Korea

• Lee Jong

  Department of Bioengineering and Technology, Kangwon National University

• Hong Eock

  Department of Bioengineering and Technology, Kangwon National University

• Park Seok

  Department of Bioengineering and Technology, Kangwon National University

• Jung Woo

  Department of Bioengineering and Technology, Kangwon National University

• LEE Keun

  Department of Materials Engineering, Yonsei University

• Lee Keun

  Department of Bioengineering and Technology, Kangwon National University

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