組織培養によるミシマサイコの繁殖

概要

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Successful asexual propagation of Bupleurum falcatum L. (Umbelliferae), an allogamous medicinal plant, was achieved through somatic embryogenesis of callus tissues derived from young leaves. When leaf explant, forming callus tissue, was kept on Linsmaier-Skoog's medium supplemented with 1 μM 2,4-dichlorophenoxyacetic acid for more than 6 months, somatic embryos of globular or heart-shaped stage differentiated on the surface of the callus tissue. The embryoids proliferated in the liquid medium containing no growth regulators. When transferred to LS medium which is reduced in strength by half, containing 1% sucrose, they grew to plantlets, which were then transplanted to soil. They grew satisfactorily to mature plants in the field. All the leaf explants taken from sterile redifferentiated plantlets gave numerous embryoids within 1 month when incubated on LS medium supplemented with 1 μM 2,4-D.
日本生薬学会の論文
1983-03-20