Pa-243 セルラーゼーリグニン複合体によるセルロース糖化機構(ポスターセッション1:2.技術,研究発表,(ポスター発表))

概要

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Cellulose is known to be bound to lignin during enzymatic saccharification of lignocellulosic biomass. Cellulase in saccharification liquor can be recovered by the addition of new cellulosic substrate, whereas it is unclear how lignin-binding cellulase is recovered. In this study, cellulase-lignocresol complex was selected as a model compound of lignin-binding cellulase. A filter paper was contacted to cellulase-lignocresol complex at room temperature for 10 to 60 min. Then, the filter paper was treated in acetate buffer at 50℃ for 24 hrs. The longer the contact time is, the more glucose was produced. However, most of the enzymatic activity still remained in the cellulase-lignocresol complex even after the contact with a filter paper. Therefore it was estimated that most cellulase is working as an immobilized cellulase at least at the beginning of saccharification. Developing a pretreatment method to make lignin inactive to cellulase and the use of cellulosic substrate with larger surface area would be effective to inhibit cellulase binding on lignin and to enable the recovery of the enzymatic activity.
2012-01-18