生薬学領域におけるマイクロテクニックの研究(I) : アラビヤゴムを永久プレパラートの封入剤に利用する
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概要
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Canada balsam xylene solution that already is used for permanent preparation is a solvent of essential oil, fat, fatty oil, resin, certain pigments and other containing substances in cells; moreover, concerning some dyes in soluble in organic solvent, the xylene solution can not be used for the histochemical studies, especially for the pharmacognostical studies. Therefore we contrived as follows: Gum Arabic-Sugar Solution (G-S) mounting agent 1. Recipe; Gum arabic (mass or broken pieces)…10 g Sucrose (cane sugar)…2 g Water…proper quantity Gum arabic and sucrose are added to proper quantity of water and heat it slightly and then make the mucilage of gum arabic. It this mucilage during the preparating procedure is concentrated solution, again add water to the same viscosity of canada balsam xylene solution. 2. Use; All the same to the mounting method already used for the canada balsam xylene solution, but the dehydration agent is disused in the case of this G-S. 3. Strong point; (1) It dose not bring contraction of cells or tissues according to no dehydrating action. (2) Some containing substances in cell are removed due to the organic solvent, but in this method these are not removed. (3) Some dyes soluble in the organic solvents can be used in this method. (4) The method and procedure are brief. (5) It is colourless for a long time as the permanent preparation. (6) It can be lightly withdrawn. (7) But in this method, it is less transparent than when the xylene solution is used, because of no use of the clearing agents. And this brings better results for the microscopical photograph, for in microscopy for the pharmacognostical studies, this magnification is under 1000 times, mostly 800 times.
- 日本生薬学会の論文
- 1958-03-25
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