A novel allele of the Arabidopsis phytochelatin synthase 1 gene conferring high sensitivity to arsenic and antimony(Plant Nutrition)
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概要
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To identify genes involved in arsenite [As(III)] tolerance, we screened for As(III)-sensitive mutants using ethyl methanesulfonate (EMS)-mutagenized seeds of Arabidopsis thaliana. One mutant with high sensitivity to As(III) was isolated from the screening. It had a mutation in Glu^<52> of phytochelatin synthase 1 (AtPCS1), and introduction of the wild-type AtPCS1 gene rescued the phenotype, confirming that the mutant represented a new allele of atpcs1 (termed as cad1-7). The expression of AtPCS1 from cad1-7 and cad1-3, a second allele of atpcs1, complemented As-sensitive yeast, but not plants. Our study suggests that AtPCS1 activity is differentially regulated between plants and yeast.
著者
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Fujiwara Toru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Kamiya Takehiro
Biotechnology Res. Center The Univ. Of Tokyo
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KAMIYA Takehiro
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Fujiwara Toru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Kamiya Takehiro
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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