日本薬局方収載のTLCによる生薬の確認試験について(1) : 1-ブタノール/水/酢酸(100)混液を展開溶媒とする確認試験

概要

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The crude drug identification tests which use a 1-butanol/water/acetic acid solvent system for TLC in the Japanese Pharmacopoeia were examined. Comparison of TLC chromatograms from 7 laboratories, using commercially available TLC plates purchased from Merck & Co. and Wako Pure Chemical Industries, revealed that in some cases the R_f values of the indicator spots were markedly influenced by the make of TLC plates, and in the tests of Lycium Bark and Plantago Herb, the R_f values observed were different from those indicated in the Japanese Pharmacopoeia. TLC chromatograms developed in two different lengths (7cm and 10cm) showed almost identical patterns for all the 12 identification tests examined. This indicates that it is possible to change the development length from 10cm to 7cm, which will save the time required for the development by about 45%, without affecting the results of the identification tests.
2011-02-20