Compressive force inhibits adipogenesis through COX-2-mediated down-regulation of PPARγ2 and C/EBPα(CELL AND TISSUE ENGINEERING)
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概要
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Various mechanical stimuli affect differentiation of mesoderm-derived cells such as osteoblasts or myoblasts, suggesting that adipogenesis may also be influenced by mechanical stimulation. However, effects of mechanical stimuli on adipogenesis are scarcely known. Compressive force was applied to a human preadipocyte cell line, SGBS. Levels of gene expression were estimated by real-time reverse transcription-polymerase chain reaction. The accumulation of lipids was evaluated by Sudan III or Oil Red O staining. In SGBS cells subjected to a compressive force of 226Pa for 12h before adipogenic induction, adipogenesis was inhibited. Compressive force immediately after adipogenic induction did not affect the adipogenesis. The expression of peroxisome proliferator-activated receptor (PPAR) γ2 and CCAAT/enhancer binding protein (C/EBP) α mRNA during adipogenesis was inhibited by compressive force, whereas C/EBPβ and C/EBPδ mRNA levels were unaffected. In preadipocytes, compressive force increased mRNA levels of Kruppel-like factor 2, preadipocyte factor 1, WNT10b, and cyclooxygenase-2 (COX-2) which are known as negative regulators for the PPARγ2 and C/EBPα genes. Furthermore, a COX-2 inhibitor completely reversed the inhibition of adipogenesis by compressive force. In conclusion, compressive force inhibited adipogenesis by suppressing expression of PPARγ2 and C/EBPα in a COX-2-dependent manner.
著者
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Hossain Md.
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Gra
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Iwata Takeo
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Gra
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Mizusawa Noriko
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Gra
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Shima Shahidan
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Gra
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Okutsu Toru
Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, The University
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Ishimoto Kyoko
Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The Univers
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Yoshimoto Katsuhiko
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Gra
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Shima Shahidan
Department Of Medical Pharmacology Institute Of Health Biosciences The University Of Tokushima Gradu
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Mizusawa Noriko
Department Of Biochemistry Iwate Medical University
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Ishimoto Kyoko
Department Of Orthodontics And Dentofacial Orthopedics Institute Of Health Biosciences The Universit
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Iwata Takeo
Department Of Medical Pharmacology Institute Of Health Biosciences The University Of Tokushima Gradu
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Iwata Takeo
Department Of Biology School Of Education Waseda University
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Okutsu Toru
Department Of Oral And Maxillofacial Prosthodontics Institute Of Health Biosciences The University O
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Yoshimoto Katsuhiko
Department Of Medical Pharmacology Institute Of Health Biosciences The University Of Tokushima Gradu
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Hossain Md.
Department Of Medical Pharmacology Institute Of Health Biosciences The University Of Tokushima Gradu
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Hossain Md.
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Mizusawa Noriko
Department Of Medical Pharmacology Institute Of Health Biosciences The University Of Tokushima Gradu
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- Compressive force inhibits adipogenesis through COX-2-mediated down-regulation of PPARγ2 and C/EBPα
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