A mass separation of chondrocytes from cartilage tissue utilizing an automatic crushing device(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
Porcine articular cartilage tissue was crushed using Multi-Beads-Shocker^<TM> and digested with trypsin and collagenase type II. Chondrocyte cell yield was maximized by automatic crushing for 10s, which was 4 times that by manual dicing with a surgical blade and might be useful for the automation of the cell processing.
著者
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Wakitani Shigeyuki
Orthopedic Surgery, Osaka City University School of Medicine
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Takagi Mutsumi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Yoshioka Hiromichi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Wakitani Shigeyuki
Orthopedic Surgery Osaka City Univ. School Of Medicine 1-4-3 Asahi-machi Abeno-ku Osaka Osaka 545-85
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Takagi Mutsumi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Yoshioka Hiromichi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Takagi Mutsumi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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TAKAGI Mutsumi
Division of Biotechnology and Macromalecular Chemistry, Graduate School of Engineering, Hokkaido University
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