Effect of Monosaccharides Composing Glycosaminoglycans on Type 2 Collagen Accumulation in a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
The effect of the addition of monosaccharides composing glycosaminoglycans (GAGs) on the accumulation of type 2 collagen (COL(II)) in a three-dimensional (3D) culture of porcine chondrocyte cells was investigated for possible application to cartilage regenerative medicine. Primary chondrocytes from porcine cartilage were cultivated in three-dimension employing atelo collagen gel for 3 weeks with the addition of several saccharides. The addition of D-glucuronic acid (D-GlcA), N-acetyl-D-galactosamine (D-GalNAc), chondroitin sulfate C (CSC), D-galactose, N-acetyl-D-glucosamine, and L-iduronic acid increased markedly not aggrecan but COL(II) accumulation although the addition of D-fructose and D-mannose not composing GAGs did not show such an effect. The addition of D-GlcA and D-GalNAc had no synergistic effect. The addition of CSC, D-GlcA, and D-GalNAc also increased COL(II) mRNA expression while aggrecan mRNA expression was not increased by these compounds. In conclusion, the addition of monosaccharides composing GAGs might be valuable for increasing COL(II) accumulation in the 3D culture of chondrocytes.
著者
-
Takagi Mutsumi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
-
Wakitani Shigeyuki
Orthopedic Surgery Osaka City Univ. School Of Medicine 1-4-3 Asahi-machi Abeno-ku Osaka Osaka 545-85
-
Wakitani Shigeyuki
Orthopedic Surgery Osaka City University School Of Medicine
-
Takagi Mutsumi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
-
Kagita Erina
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
-
Ikeda Masahiro
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
-
Ikeda Masahiro
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
-
Kagita Erina
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
-
Takagi Mutsumi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
-
TAKAGI Mutsumi
Division of Biotechnology and Macromalecular Chemistry, Graduate School of Engineering, Hokkaido University
関連論文
- Noninvasive discrimination of human normal cells and malignant tumor cells by phase-shifting laser microscopy(CELL AND TISSUE ENGINEERING)
- Effect of Monosaccharides Composing Glycosaminoglycans on Type 2 Collagen Accumulation in a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
- A mass separation of chondrocytes from cartilage tissue utilizing an automatic crushing device(CELL AND TISSUE ENGINEERING)
- Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum(CELL AND TISSUE ENGINEERING)
- Influence of Fetal Calf Serum on Differentiation of Mesenchymal Stem Cells to Chondrocytes during Expansion(CELL AND TISSUE ENGINEERING)
- Combination of cytomegalovirus enhancer with human cellular promoters for gene-induced chondrogenesis of human bone marrow mesenchymal stem cells(CELL AND TISSUE ENGINEERING)
- Cell Processing Engineering for Ex-Vivo Expansion of Hematopoietic Cells
- Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
- Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
- Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length
- Effect of Poly DL-Lactic-Co-Glycolic Acid Mesh on a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
- Effect of Static Pressure on Intracellular pH of Adhesive Chinese Hamster Ovary Cells(CELL AND TISSUE ENGINEERING)
- Development of hybrid artificial lung with gene transfected biological cells
- High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.
- Ex vivo cartilage defect model for the evaluation of cartilage regeneration using mesenchymal stem cells(CELL AND TISSUE ENGINEERING)
- Quantitative correlation between production rate of melanoma inhibitory activity and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes and redifferentiation of chondrocytes(CELL AND TISSUE ENGINEERING)
- Shrinkage-free preparation of scaffold-free cartilage-like disk-shaped cell sheet using human bone marrow mesenchymal stem cells(CELL AND TISSUE ENGINEERING)
- Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells(CELL AND TISSUE ENGINEERING)
- Correlation between actin content and laser phase shift of adhesive normal and malignant prostate epithelial cells(CELL AND TISSUE ENGINEERING)
- Xeno-free and shrinkage-free preparation of scaffold-free cartilage-like disc-shaped cell sheet using human bone marrow mesenchymal stem cells(CELL AND TISSUE ENGINEERING)