High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.
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概要
- 論文の詳細を見る
The effects of the density of human mesenchymal stem cells (MSCs) on their differentioation to chondrocytes in a differentiation medium supplemented with dexamethasone, TGFβ3, and IGF-1 were investigated for the regenerative therapy of cartilage. The increase in the initial density of MSCs from 0.05×104 to 0.9×104 cells/cm2 accelerated the increase in the expression level of aggrecan mRNA during the differentiation culture for 7 d. The conditioned medium harvested at 7 d from the differentiation culture with an initial MSC density of 0.3×104 cells/cm2 accelerated the initial increase in the expression level for 3 d in the differentiation culture with an initial MSC density of 0.3×104 cells/cm2, whereas the conditioned medium harvested at 7 d in the differentiation culture with an initial MSC density of 0.05×104 cells/cm2 did not. The differentiation culture after 14 d with an initial MSC concentration of 0.3×104 cells/cm2 showed an expression level 1.7-fold that in the case of the culture with an initial MSC concentration of 0.05×104 cells/cm2. Thus, a high MSC inoculum density might be appropriate for the rapid differentiation of MSCs to chondrocytes.
- 社団法人日本生物工学会の論文
- 2007-01-25
著者
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Takagi Mutsumi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Fujiwara Masashi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Wakitani Shigeyuki
Orthopedic Surgery Osaka City Univ. School Of Medicine 1-4-3 Asahi-machi Abeno-ku Osaka Osaka 545-85
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Takagi Mutsumi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Fujiwara Masashi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Umetsu Yousuke
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Umetsu Yousuke
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Takagi Mutsumi
Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University
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