Methylation法およびTranscription法を用いたOligodendrogliomaにおけるClonality Analysis

概要

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Characterization of the clonal derivation of human neoplasms has provided important information about the etiology and pathogenesis, and has oractical implications for both the diagnosis and subsequent studies on disease progression. ln the oresent study, molecular srenetic assays, including methylation- and transcription-based donal techniques were used to determine the clonality of 13 oligodendroglioma oatients (16 samples). Analysis of the tumors with PCR-based methylation assays at the androgen receotor locus (HUMARA)confirmed that three informative benign sarrmles and two of eight malignant sairmles were monoclonal, and the remaining six malignant samples were all polyclonal. Of three patients who had two separate operations, two consistently showed both monoclonal, and one showed monoclonal at the first operation, then changed to polyclonal. Transcription-based analysis showed four informative benign samples and three of six were malignant samples were monoclonal, while the remaining three were polyclonal. Two samples, which showed polyclonal findings by methylation-based analysis, were demonstrated to be monoclonal by transcription-based analysis. Our data demonstrated that many tumors consist of homogeneous clonal cells in origin but some are mixed, showing both heterogeneous cells and other populations of clonal cells.
東京女子医科大学の論文
1999-05-25