MICRO-ASSAY METHOD FOR VASOPRESSIN BY THIN-LAYER CHROMATOGRAPHY
スポンサーリンク
概要
- 論文の詳細を見る
A method for the micro-assay of vasopressin by thin-layer chromatography was proposed. Vasopressin was subjected to trypsin digestion, and the released glycinamide was developed on a polyamide sheet after modification with densyl chloride. The fluorescence intensity of the densyl glycinamide was quantitated by a thin-layer chromatoscanner with an excitation wave length at 260 nm, and the emission wave length at 500 nm. The lowest limit by this estimation method was 100 ng of vasopressin. The pressor units of L8-Vasopressin and Pitressin were 115 U/mg and 482 U/mg, respectively. The method presented here is useful for chemical assay of a large number of vasopressin preparations in a short time.
- 名古屋市立大学の論文
- 1979-11-30
著者
-
佐々木 実
Department Of Biochemistry Nagoya City University Medical School
-
加藤 泰治
Department Of Biochemistry Nagoya City University Medical School
-
久田 四郎
Department Of Pharmacology Nagoya City University Medical School
-
津島 宏美
Department of Pharmacology, Nagoya City University Medical School
-
藤本 征五
Department of Pharmacology, Nagoya City University Medical School
-
藤本 征五
名古屋市立大学 産婦人科
-
津島 宏美
Department Of Pharmacology Nagoya City University Medical School
-
藤本 征五
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University
関連論文
- 高血圧自然発症ラットの動脈筋小胞体のカルシウム保持能の異常
- MICRO-ASSAY METHOD FOR VASOPRESSIN BY THIN-LAYER CHROMATOGRAPHY
- EFFECTS OF INTRACEREBROVENTRICULAR MORPHINE AND ENKEPHALINS ON THE URINE OUTFLOW IN ETHANOL-ANAESTHETIZED RATS
- KINETIC STUDIES OF ANTIGEN-ANTIBODY REACTION BETWEEN FRUIT BROMELAIN AND ITS UNIVALENT ANTIBODY BY MEANS OF FLUOROMETRY
- INHIBITION OF FRUIT BROMELAIN BY ITS UNIVALENT ANTIBODIES CROSS-REACTING AND NOT CROSS-REACTING WITH STEM BROMELAIN
- AN ACUTE AND FOCAL OSTEOPENIA MODEL USING OVARIECTOMIZED RATS : A RAPID DETECTION OF THE PROTECTIVE EFFECT OF SALMON CALCITONIN
- 免疫学的手法の利用--系統発生学的に関連のある植物酵素の免疫学的解析 (植物酵素・蛋白質研究法) -- (植物酵素・蛋白質の精製法と各種研究法)
- ダンシル蛍光標識タンパク質・ペプチドのSDSゲル電気泳動--分子量の測定と化学分析への応用(実験講座)
- 蛍光法によるタンパク質抗原とその抗体間の新和力測定法 (蛍光測定の生化学研究への応用)
- TBE, OR NOT TBE ; THAT IS THE QUESTION : Beneficial Usage of Tris-Borate for Obtaining a Higher Resolution of Small DNA Fragments by Agarose Gel Electrophoresis
- HYPOTHALAMIC PROSTAGLANDINS AND WATER METABOLISM A REVIEW
- ANTIDIURETIC HORMONE AND THE HYPOTHALAMO-NEUROHYPOPHYSEAL SYSTEM : A REVIEW
- 54) 高血圧自然発症ラット(SHR)の動脈におけるβ_1, β_2弛緩反応の減弱 : 日本循環器会第71回東海・第56回北陸合同地方会
- Ouabain不整脈にたいするTaurineの拮抗作用について
- EFFECT OF COMPOUNDS AFFECTING THE ADRENERGIC MECHANISM ON CELL GROWTH AND DIVISION OF TETRAHYMENA PYRIFORMIS W
- VARIANCE OF THE GROWTH OF TETRAHYMENA BY THE COMPOUNDS WHICH AFFECT ON THE ADRENERGIC MECHANISM
- 視床下部におけるProstaglandinsの意義
- 視床下―下垂体系と抗利尿ホルモン