TBE, OR NOT TBE ; THAT IS THE QUESTION : Beneficial Usage of Tris-Borate for Obtaining a Higher Resolution of Small DNA Fragments by Agarose Gel Electrophoresis

スポンサーリンク

概要

• 論文の詳細を見る

• Tris-borate buffer (TBE) was advantageous for obtaining a higher resolution of smaller DNA fragments on agarose gels, when compared to the conventional tris-acetate buffer (TAE). Critical DNA sizes and gel concentrations for a clear separation were about 2-kb for the 0.8%agarose and 300-bp for the 2.0%agarose. DNA fragments larger than the critical size (>2-kb on 0.8%agarose gel) migrate faster in TAE, and the smaller fragments (<300-bp on 2% agarose gel) migrate faster in TBE showing the better resolution in a mini chamber system (Mupid^<TM>-21).

• 名古屋市立大学の論文
• 1999-07-31

著者

• 加藤 泰治

  Department Of Biochemistry Nagoya City University Medical School

• 加藤 泰治

  Department Of Bioregulation Research Nagoya City University

• 三浦 裕

  Department Of Bioregulation Research Nagoya City University

• 和気 弘明

  Department of Bioregulation Research, Nagoya City University

• 和気 弘明

  Department Of Bioregulation Research Nagoya City University

関連論文

• 転写調節因子ATBF1mRNAの肺癌での発現の変化
• 血液・脳関門機能とアストロサイト
• ニュ-ロン・グリア相関におけるグリオスタチンの役割 (神経栄養因子--新しい展開)
• MICRO-ASSAY METHOD FOR VASOPRESSIN BY THIN-LAYER CHROMATOGRAPHY
• KINETIC STUDIES OF ANTIGEN-ANTIBODY REACTION BETWEEN FRUIT BROMELAIN AND ITS UNIVALENT ANTIBODY BY MEANS OF FLUOROMETRY
• INHIBITION OF FRUIT BROMELAIN BY ITS UNIVALENT ANTIBODIES CROSS-REACTING AND NOT CROSS-REACTING WITH STEM BROMELAIN
• FUNCTIONAL ANALYSIS OF ATBF1,A MULTIPLE-HOMEODOMAIN ZINC FINGER PROTEIN, USING LUNG CANCER CELL LINE A549
• 免疫学的手法の利用--系統発生学的に関連のある植物酵素の免疫学的解析 (植物酵素・蛋白質研究法) -- (植物酵素・蛋白質の精製法と各種研究法)
• ダンシル蛍光標識タンパク質・ペプチドのSDSゲル電気泳動--分子量の測定と化学分析への応用(実験講座)
• 蛍光法によるタンパク質抗原とその抗体間の新和力測定法 (蛍光測定の生化学研究への応用)
• TBE, OR NOT TBE ; THAT IS THE QUESTION : Beneficial Usage of Tris-Borate for Obtaining a Higher Resolution of Small DNA Fragments by Agarose Gel Electrophoresis
• ATBF1の転写制御ドメインのマッピング

もっと見る 閉じる

スポンサーリンク